{"created":"2024-10-30T09:22:49.019897+00:00","id":2007426,"links":{},"metadata":{"_buckets":{"deposit":"2d68f249-20e2-4e92-9dbf-221dd5e8dbcc"},"_deposit":{"created_by":7,"id":"2007426","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2007426"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02007426","sets":["1713853213384:1713853295607"]},"author_link":["748","664"],"control_number":"2007426","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Multiplex Genome Editing in Tomato","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-07-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"916","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"Frontiers in Plant Science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Several expression systems for multiple guide RNA (gRNAs) have been developed in the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) system to induce multiple-gene modifications in plants. Here, we evaluated mutation efficiencies in the tomato genome using multiplex CRISPR/Cas9 vectors consisting of various Cas9 expression promoters with multiple gRNA expression combinations. In transgenic tomato calli induced with these vectors, mutation patterns varied depending on the promoters used to express Cas9. By using the tomato ELONGATION FACTOR-1α (SlEF1α) promoter to drive Cas9, occurrence of various types of mutations with high efficiency was detected in the tomato genome. Furthermore, sequence analysis showed that the majority of mutations using the multiplex system with the SlEF1α promoter corresponded to specific mutation pattern of deletions produced by self-ligation at two target sites of CRISPR/Cas9 with low mosaic mutations. These results suggest that optimizing the Cas9 expression promoter used in CRISPR/Cas9-mediated mutation improves multiplex genome editing, and could be used effectively to disrupt functional domains precisely in the tomato genome.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Frontiers Media SA","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"Copyright © 2018 Hashimoto, Ueta, Abe, Osakabe and Osakabe. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"1664462X","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.3389/fpls.2018.00916"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3389/fpls.2018.00916","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"340086"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ハシモト, リョウスケ","creatorNameLang":"ja"},{"creatorName":"ハシモト, リョウスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Hashimoto, Ryosuke","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ウエタ, リサ","creatorNameLang":"ja"},{"creatorName":"ウエタ, リサ","creatorNameLang":"ja-Kana"},{"creatorName":"Ueta, Risa","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"アベ, チヒロ","creatorNameLang":"ja"},{"creatorName":"アベ, チヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Abe, Chihiro","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"刑部, 祐里子","creatorNameLang":"ja"},{"creatorName":"オサカベ, ユリコ","creatorNameLang":"ja-Kana"},{"creatorName":"Osakabe, Yuriko","creatorNameLang":"en"}],"familyNames":[{"familyName":"刑部","familyNameLang":"ja"},{"familyName":"オサカベ","familyNameLang":"ja-Kana"},{"familyName":"Osakabe","familyNameLang":"en"}],"givenNames":[{"givenName":"祐里子","givenNameLang":"ja"},{"givenName":"ユリコ","givenNameLang":"ja-Kana"},{"givenName":"Yuriko","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"748","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50444071","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=50444071"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"刑部, 敬史","creatorNameLang":"ja"},{"creatorName":"オサカベ, ケイシ","creatorNameLang":"ja-Kana"},{"creatorName":"Osakabe, Keishi","creatorNameLang":"en"}],"familyNames":[{"familyName":"刑部","familyNameLang":"ja"},{"familyName":"オサカベ","familyNameLang":"ja-Kana"},{"familyName":"Osakabe","familyNameLang":"en"}],"givenNames":[{"givenName":"敬史","givenNameLang":"ja"},{"givenName":"ケイシ","givenNameLang":"ja-Kana"},{"givenName":"Keishi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"664","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"272329/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/272329/profile-ja.html"},{"nameIdentifier":"70450335","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=70450335"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2020-11-19"}],"displaytype":"detail","filename":"fpls_9_916.pdf","filesize":[{"value":"2.89 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2007426/files/fpls_9_916.pdf"},"version_id":"beeb352f-bcc1-4410-838a-1b707d622dcb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CRISPR/Cas9","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"multiplex genome editing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SlEF1α promoter","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tomato","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Efficient Multiplex Genome Editing Induces Precise, and Self-Ligated Type Mutations in Tomato Plants","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Efficient Multiplex Genome Editing Induces Precise, and Self-Ligated Type Mutations in Tomato Plants","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-11-19"},"publish_date":"2020-11-19","publish_status":"0","recid":"2007426","relation_version_is_last":true,"title":["Efficient Multiplex Genome Editing Induces Precise, and Self-Ligated Type Mutations in Tomato Plants"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-06-06T00:11:18.426637+00:00"}