{"created":"2024-10-30T09:34:39.257392+00:00","id":2007568,"links":{},"metadata":{"_buckets":{"deposit":"fba38044-7c7b-4859-8dba-abe9ba08159a"},"_deposit":{"created_by":7,"id":"2007568","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2007568"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02007568","sets":["1713853213384:1713853296295:1716267878536:1716268699858"]},"author_link":["516","567","1234","1579"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"腸炎ビブリオ3型分泌装置エフェクタータンパク質VopQは、上皮細胞の糖代謝を変化させる","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"V. parahaemolyticus effector modulates host metabolism","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-03-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageStart":"e00960-19","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"mSphere","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Vibrio parahaemolyticus is a Gram-negative halophilic pathogen that frequently causing acute gastroenteritis and occasional wound infection. V. parahaemolyticus contains several virulent factors, including Type III secretion systems (T3SSs) and thermostable direct hemolysin (TDH). In particular, T3SS1 is a potent cytotoxic inducer, and T3SS2 is essential for causing acute gastroenteritis. Although much is known about V. parahaemolyticus’s effector manipulating host signaling transductions, little is known about the host metabolomic changes modulated by V. parahaemolyticus. To address this knowledge gap, we performed a metabolomic analysis of the epithelial cells during V. parahaemolyticus infection using capillary electrophoresis–time-of-flight mass spectrometry (CE-TOF/MS). Our results revealed significant metabolomic perturbations upon V. parahaemolyticus infection. Moreover, we identified that T3SS1’s VopQ effector was responsible for inducing the significant metabolic changes in the infected cells. The VopQ effector dramatically altered the host cell’s glycolytic, tricarboxylic acid cycle (TCA), amino acid metabolisms. VopQ effector disrupted host cell redox homeostasis by depleting cellular glutathione and subsequently increasing the level of reactive oxygen species (ROS) production.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2020 Nguyen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.( https://creativecommons.org/licenses/by/4.0/ )","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"23795042","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3413号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（栄養学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2020-03-12"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開\n内容要旨・審査要旨：k3413_abstract_review.pdf\n論文本文：k3413_fulltext.pdf (著者最終稿)\n論文本文：msphere_5_2_e00960-19.pdf (雑誌掲載版)\n本論文は, 著者Nguyen Quoc Anhの学位論文として提出され, 学位審査・授与の対象となっている。\n学位授与者所属 : 徳島大学大学院栄養生命科学教育部（人間栄養科学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲栄第274号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1128/mSphere.00960-19"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1128/mSphere.00960-19","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"364047"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"グェン, クォ アイン","creatorNameLang":"ja"},{"creatorName":"グェン, クォ アイン","creatorNameLang":"ja-Kana"},{"creatorName":"Nguyen, Quoc Anh","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"下畑, 隆明","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"516","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ハタヤマ, ショウ","creatorNameLang":"ja"},{"creatorName":"ハタヤマ, ショウ","creatorNameLang":"ja-Kana"},{"creatorName":"Hatayama, Sho","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"テンタク, アヤ","creatorNameLang":"ja"},{"creatorName":"テンタク, アヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Tentaku, Aya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"キド, ジュンコ","creatorNameLang":"ja"},{"creatorName":"キド, ジュンコ","creatorNameLang":"ja-Kana"},{"creatorName":"Kido, Junko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Huong Bui, Thi Mai","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"上番増, 喬","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"567","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"馬渡, 一諭","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1234","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"髙橋, 章","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1579","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2020-04-27"}],"displaytype":"detail","filename":"msphere_5_2_e00960-19.pdf","filesize":[{"value":"3.33 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2007568/files/msphere_5_2_e00960-19.pdf"},"version_id":"50490f56-4ad5-4dae-a673-02f6ded1409f"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2020-04-27"}],"displaytype":"detail","filename":"k3413_abstract_review.pdf","filesize":[{"value":"174 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parahaemolyticus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"T3SS effector","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Type III Secretion Effector VopQ of Vibrio parahaemolyticus Modulates Epithelial Cells’ Central Carbon Metabolism","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Type III Secretion Effector VopQ of Vibrio parahaemolyticus Modulates Epithelial Cells’ Central Carbon Metabolism","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268699858"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-04-27"},"publish_date":"2020-04-27","publish_status":"0","recid":"2007568","relation_version_is_last":true,"title":["Type III Secretion Effector VopQ of Vibrio parahaemolyticus Modulates Epithelial Cells’ Central Carbon Metabolism"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-30T09:34:49.958431+00:00"}