{"created":"2024-10-30T09:47:02.386550+00:00","id":2007707,"links":{},"metadata":{"_buckets":{"deposit":"2e64904a-96ef-4687-b67e-29260230948b"},"_deposit":{"created_by":7,"id":"2007707","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2007707"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02007707","sets":["1713853213384:1713853295607"]},"author_link":["807","549"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-05-29","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"113390","bibliographicVolumeNumber":"188","bibliographic_titles":[{"bibliographic_title":"Journal of Pharmaceutical and Biomedical Analysis","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Competitive inhibition between two substrates with an enzyme is investigated by capillary electrophoresis/dynamic frontal analysis (CE/DFA). Enzymatic hydrolyses of o-nitrophenyl β-D-galactopyranoside and p-nitrophenyl β-D-galactopyranoside with β-D-galactosidase were examined as a model competitive reaction. A sample solution containing the two substrates was injected into a capillary filled with a separation buffer containing an enzyme. Enzymatic hydrolysis occurred during the electrophoresis, and the products of o-nitrophenol and p-nitrophenol were continuously formed and resolved from the sample zone. Two-steps plateau signal was detected with the two-substrate solutions based on the difference in the effective electrophoretic mobility of o-nitrophenol and p-nitrophenol. Michaelis-Menten constants and inhibition constants were determined with the plateau heights. Usefulness of CE/DFA on competitive inhibition analysis is demonstrated in this study.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"07317085","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA10644220","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA11533870","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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Toshio","creatorNameLang":"en"}],"familyNames":[{"familyName":"髙栁","familyNameLang":"ja"},{"familyName":"タカヤナギ","familyNameLang":"ja-Kana"},{"familyName":"Takayanagi","familyNameLang":"en"}],"givenNames":[{"givenName":"俊夫","givenNameLang":"ja"},{"givenName":"トシオ","givenNameLang":"ja-Kana"},{"givenName":"Toshio","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"549","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"241977/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/241977/profile-ja.html"},{"nameIdentifier":"50263554","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=50263554"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-05-29"}],"displaytype":"detail","filename":"jpba_188_113390.pdf","filesize":[{"value":"978 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constant","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Kinetic analysis of substrate competition in enzymatic reactions with β-D-galactosidase by capillary electrophoresis / dynamic frontal analysis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Kinetic analysis of substrate competition in enzymatic reactions with β-D-galactosidase by capillary electrophoresis / dynamic frontal analysis","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-06-15"},"publish_date":"2020-06-15","publish_status":"0","recid":"2007707","relation_version_is_last":true,"title":["Kinetic analysis of substrate competition in enzymatic reactions with β-D-galactosidase by capillary electrophoresis / dynamic frontal analysis"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-29T13:58:08.137776+00:00"}