{"created":"2024-10-30T09:58:55.716044+00:00","id":2007865,"links":{},"metadata":{"_buckets":{"deposit":"99b4be30-0da6-4d39-8c9e-b48038b76fb5"},"_deposit":{"created_by":7,"id":"2007865","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2007865"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02007865","sets":["1713853213384:1713853295607"]},"author_link":["1116"],"control_number":"2007865","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Roles of Phosphatidic Acid in Virus RNA Replication","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-05-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageStart":"e1004909","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"PLOS Pathogens","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Eukaryotic positive-strand RNA [(+)RNA] viruses are intracellular obligate parasites replicate using the membrane-bound replicase complexes that contain multiple viral and host components. To replicate, (+)RNA viruses exploit host resources and modify host metabolism and membrane organization. Phospholipase D (PLD) is a phosphatidylcholine- and phosphatidylethanolamine-hydrolyzing enzyme that catalyzes the production of phosphatidic acid (PA), a lipid second messenger that modulates diverse intracellular signaling in various organisms. PA is normally present in small amounts (less than 1% of total phospholipids), but rapidly and transiently accumulates in lipid bilayers in response to different environmental cues such as biotic and abiotic stresses in plants. However, the precise functions of PLD and PA remain unknown. Here, we report the roles of PLD and PA in genomic RNA replication of a plant (+)RNA virus, Red clover necrotic mosaic virus (RCNMV). We found that RCNMV RNA replication complexes formed in Nicotiana benthamiana contained PLDα and PLDβ. Gene-silencing and pharmacological inhibition approaches showed that PLDs and PLDs-derived PA are required for viral RNA replication. Consistent with this, exogenous application of PA enhanced viral RNA replication in plant cells and plant-derived cell-free extracts. We also found that a viral auxiliary replication protein bound to PA in vitro, and that the amount of PA increased in RCNMV-infected plant leaves. Together, our findings suggest that RCNMV hijacks host PA-producing enzymes to replicate.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"PLOS","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2015 Hyodo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"15537366","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"15537374","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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ユウキ","creatorNameLang":"ja"},{"creatorName":"マナベ, ユウキ","creatorNameLang":"ja-Kana"},{"creatorName":"Manabe, Yuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"カイドウ, マサノリ","creatorNameLang":"ja"},{"creatorName":"カイドウ, マサノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Kaido, Masanori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ミセ, カズユキ","creatorNameLang":"ja"},{"creatorName":"ミセ, カズユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Mise, Kazuyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"スガワラ, タツヤ","creatorNameLang":"ja"},{"creatorName":"スガワラ, タツヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Sugawara, Tatsuya","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"谷口, 寿章","creatorNameLang":"ja"},{"creatorName":"タニグチ, ヒサアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Taniguchi, Hisaaki","creatorNameLang":"en"}],"familyNames":[{"familyName":"谷口","familyNameLang":"ja"},{"familyName":"タニグチ","familyNameLang":"ja-Kana"},{"familyName":"Taniguchi","familyNameLang":"en"}],"givenNames":[{"givenName":"寿章","givenNameLang":"ja"},{"givenName":"ヒサアキ","givenNameLang":"ja-Kana"},{"givenName":"Hisaaki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1116","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60596/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/60596/profile-ja.html"},{"nameIdentifier":"10257636","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=10257636"}]},{"creatorNames":[{"creatorName":"オクノ, テツロウ","creatorNameLang":"ja"},{"creatorName":"オクノ, テツロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Okuno, 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