{"created":"2024-10-30T10:07:05.282761+00:00","id":2007965,"links":{},"metadata":{"_buckets":{"deposit":"77e83690-88fa-4073-b252-b2958fed1378"},"_deposit":{"created_by":7,"id":"2007965","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2007965"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02007965","sets":["1713853213384:1713853295607"]},"author_link":["1145"],"control_number":"2007965","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-11-09","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"231","bibliographicPageStart":"223","bibliographicVolumeNumber":"400","bibliographic_titles":[{"bibliographic_title":"Cancer Letters","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Metronomic chemotherapy is currently considered an emerging therapeutic option in clinical oncology. S-1, an oral formulation of Tegafur (TF), a prodrug of 5-fluorouracil (5-FU), is designed to improve the antitumor activity of 5-FU in tandem with reducing its toxicity. Clinically, metronomic S-1 dosing has been approved for the standard first- and second-line treatment of metastatic or advanced stage of colorectal (CRC). However, expression of intratumor thymidylate synthase (TS), a significant gene in cellular proliferation, is associated with poor outcome to 5-FU-based chemotherapeutic regimens. In this study, therefore, we examined the effect of a combination of TS silencing by an RNA interfering molecule, chemically synthesized short hairpin RNA against TS (shTS), and 5-FU on the growth of human colorectal cancer cell (DLD-1) both in vitro and in vivo. The combined treatment of both shTS with 5-FU substantially inhibited cell proliferation in vitro. For in vivo treatments, the combined treatment of metronomic S-1 dosing with intravenously injected polyethylene glycol (PEG)-coated shTS-lipoplex significantly suppressed tumor growth, compared to a single treatment of either S-1 or PEG-coated shTS-lipoplex. In addition, the combined treatment increased the proportion of apoptotic cells in the DLD-1 tumor tissue. Our results suggest that metronomic S-1 dosing combined with TS silencing might represent an emerging therapeutic strategy for the treatment of patients with advanced CRC.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2016 The Author(s). Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"03043835","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00598513","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA1152274X","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1016/j.canlet.2016.11.005"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.canlet.2016.11.005","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"321160"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Lila, Amr S. Abu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"モリヨシ, ナオト","creatorNameLang":"ja"},{"creatorName":"モリヨシ, ナオト","creatorNameLang":"ja-Kana"},{"creatorName":"Moriyoshi, Naoto","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"フクシマ, マサカズ","creatorNameLang":"ja"},{"creatorName":"フクシマ, マサカズ","creatorNameLang":"ja-Kana"},{"creatorName":"Fukushima, Masakazu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Huang, Cheng-Long","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ワダ, ヒロミ","creatorNameLang":"ja"},{"creatorName":"ワダ, ヒロミ","creatorNameLang":"ja-Kana"},{"creatorName":"Wada, Hiromi","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"石田, 竜弘","creatorNameLang":"ja"},{"creatorName":"イシダ, タツヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Ishida, Tatsuhiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"石田","familyNameLang":"ja"},{"familyName":"イシダ","familyNameLang":"ja-Kana"},{"familyName":"Ishida","familyNameLang":"en"}],"givenNames":[{"givenName":"竜弘","givenNameLang":"ja"},{"givenName":"タツヒロ","givenNameLang":"ja-Kana"},{"givenName":"Tatsuhiro","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1145","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60644/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/60644/profile-ja.html"},{"nameIdentifier":"50325271","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=50325271"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2020-10-26"}],"displaytype":"detail","filename":"cl_400_223.pdf","filesize":[{"value":"1.84 MB"}],"format":"application/pdf","licensetype":"license_5","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2007965/files/cl_400_223.pdf"},"version_id":"0dcdefbe-616d-4845-958f-3168cdc20979"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Colorectal cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PEG-coated shRNA-lipoplex","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RNAi","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"S-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Short hairpin RNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Thymidylate synthase","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Metronomic S-1 dosing and thymidylate synthase silencing have synergistic antitumor efficacy in a colorectal cancer xenograft model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Metronomic S-1 dosing and thymidylate synthase silencing have synergistic antitumor efficacy in a colorectal cancer xenograft model","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-10-26"},"publish_date":"2020-10-26","publish_status":"0","recid":"2007965","relation_version_is_last":true,"title":["Metronomic S-1 dosing and thymidylate synthase silencing have synergistic antitumor efficacy in a colorectal cancer xenograft model"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-30T07:50:28.506684+00:00"}