{"created":"2024-10-30T10:07:37.011276+00:00","id":2007970,"links":{},"metadata":{"_buckets":{"deposit":"983be71b-b391-4eb6-a4ed-b6a3979d5c11"},"_deposit":{"created_by":7,"id":"2007970","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2007970"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02007970","sets":["1713853213384:1713853295607"]},"author_link":["1542","468"],"control_number":"2007970","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Anti-glycopeptide mAb LpMab-21 against Podoplanin","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-01-19","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"396","bibliographicPageStart":"382","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"Cancer Medicine","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Human podoplanin (hPDPN), which binds to C‐type lectin‐like receptor‐2 (CLEC‐2), is involved in platelet aggregation and cancer metastasis. The expression of hPDPN in cancer cells or cancer‐associated fibroblasts indicates poor prognosis. Human lymphatic endothelial cells, lung‐type I alveolar cells, and renal glomerular epithelial cells express hPDPN. Although numerous monoclonal antibodies (mA bs) against hPDPN are available, they recognize peptide epitopes of hPDPN. Here, we generated a novel anti‐hPDPN mA b, LpMab‐21. To characterize the hPDPN epitope recognized by the LpMab‐21, we established glycan‐deficient CHO‐S and HEK‐293T cell lines, using the CRISPR/Cas9 or TALEN. Flow cytometric analysis revealed that the minimum hPDPN epitope, in which sialic acid is linked to Thr76, recognized by LpMab‐21 is Thr76–Arg79. LpMab‐21 detected hPDPN expression in glioblastoma, oral squamous carcinoma, and seminoma cells as well as in normal lymphatic endothelial cells. However, LpMab‐21 did not react with renal glomerular epithelial cells or lung type I alveolar cells, indicating that sialylation of hPDPN Thr76 is cell‐type‐specific. LpMab‐21 combined with other anti‐hPDPN antibodies that recognize different epitopes may therefore be useful for determining the physiological function of sialylated hPDPN.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"John Wiley & Sons","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2016 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"20457634","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1002/cam4.954"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1002/cam4.954","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"327451"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"カネコ, ミカ","creatorNameLang":"ja"},{"creatorName":"カネコ, ミカ","creatorNameLang":"ja-Kana"},{"creatorName":"Kaneko, Mika K.","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ナカムラ, タクロウ","creatorNameLang":"ja"},{"creatorName":"ナカムラ, タクロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakamura, Takuro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ホンマ, リュウスケ","creatorNameLang":"ja"},{"creatorName":"ホンマ, リュウスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Honma, Ryusuke","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"オガサワラ, サトシ","creatorNameLang":"ja"},{"creatorName":"オガサワラ, サトシ","creatorNameLang":"ja-Kana"},{"creatorName":"Ogasawara, Satoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"フジイ, ユウキ","creatorNameLang":"ja"},{"creatorName":"フジイ, ユウキ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujii, Yuki","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"阿部, 真治","creatorNameLang":"ja"},{"creatorName":"アベ, シンジ","creatorNameLang":"ja-Kana"},{"creatorName":"Abe, Shinji","creatorNameLang":"en"}],"familyNames":[{"familyName":"阿部","familyNameLang":"ja"},{"familyName":"アベ","familyNameLang":"ja-Kana"},{"familyName":"Abe","familyNameLang":"en"}],"givenNames":[{"givenName":"真治","givenNameLang":"ja"},{"givenName":"シンジ","givenNameLang":"ja-Kana"},{"givenName":"Shinji","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"468","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"213642/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/213642/profile-ja.html"},{"nameIdentifier":"00403717","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=00403717"}]},{"creatorNames":[{"creatorName":"タカギ, ミチアキ","creatorNameLang":"ja"},{"creatorName":"タカギ, ミチアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Takagi, Michiaki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ハラダ, ヒロユキ","creatorNameLang":"ja"},{"creatorName":"ハラダ, 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Yasuhiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"西岡","familyNameLang":"ja"},{"familyName":"ニシオカ","familyNameLang":"ja-Kana"},{"familyName":"Nishioka","familyNameLang":"en"}],"givenNames":[{"givenName":"安彦","givenNameLang":"ja"},{"givenName":"ヤスヒコ","givenNameLang":"ja-Kana"},{"givenName":"Yasuhiko","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1542","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60062/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/60062/profile-ja.html"},{"nameIdentifier":"70274199","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=70274199"}]},{"creatorNames":[{"creatorName":"カトウ, ユキナリ","creatorNameLang":"ja"},{"creatorName":"カトウ, ユキナリ","creatorNameLang":"ja-Kana"},{"creatorName":"Kato, Yukinari","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2020-10-27"}],"displaytype":"detail","filename":"cam_6_2_382.pdf","filesize":[{"value":"1.85 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2007970/files/cam_6_2_382.pdf"},"version_id":"61afc466-4762-4332-a712-d313fb513c31"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Epitope","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"glycopeptide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"monoclonal antibody","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"podoplanin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sialic acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development and characterization of anti‐glycopeptide monoclonal antibodies against human podoplanin, using glycan‐deficient cell lines generated by CRISPR /Cas9 and TALEN","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development and characterization of anti‐glycopeptide monoclonal antibodies against human podoplanin, using glycan‐deficient cell lines generated by CRISPR /Cas9 and TALEN","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-10-27"},"publish_date":"2020-10-27","publish_status":"0","recid":"2007970","relation_version_is_last":true,"title":["Development and characterization of anti‐glycopeptide monoclonal antibodies against human podoplanin, using glycan‐deficient cell lines generated by CRISPR /Cas9 and TALEN"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-02-10T07:07:50.036640+00:00"}