| Item type |
文献 / Documents(1) |
| 公開日 |
2020-09-14 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
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|
関連識別子 |
https://doi.org/10.1016/j.jchromb.2020.122213 |
|
|
関連名称 |
10.1016/j.jchromb.2020.122213 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Isolation of glycosylinositol phosphoceramide and phytoceramide 1-phosphate in plants and their chemical stabilities |
| 著者 |
Hasi, Rumana Yesmin
マジマ, ダイ
モリト, カツヤ
Ali, Hanif
小暮, 健太朗
Nanjundan, Meera
林, 順司
川上, 竜巳
金丸, 芳
田中, 保
|
| 抄録 |
|
|
内容記述 |
Glycosylinositol phosphoceramide (GIPC) is a sphingophospholipid in plants. Recently, we identified that GIPC is hydrolyzed to phytoceramide 1-phosphate (PC1P) by an uncharacterized phospholipase D activity following homogenization of certain plant tissues. We now developed methods for isolation of GIPC and PC1P from plant tissues and characterized their chemical stabilities. Hydrophilic solvents, namely a lower layer of a mixed solvent system consisting of isopropanol/hexane/water (55:20:25, v/v/v) was efficient solvent for extraction and eluent in column chromatography. GIPC was isolated by Sephadex column chromatography followed by TLC. A conventional method, such as the Bligh and Dyer method, was applicable for PC1P extraction. Specifically, PC1P was isolated by TLC following mild alkali treatment of lipid extracts of plants. The yields of GIPC and PC1P in our methods were both around 50-70%. We found that PC1P is tolerant against heat (up to 125 °C), strong acid (up to 10 M HCl), and mild alkali (0.1 M KOH). In contrast, significant degradation of GIPC occurred at 100 °C and 1.0 M HCl treatment, suggesting the instability of the inositol glycan moiety in these conditions. These data will be useful for further biochemical and nutritional studies on these sphingolipids. |
| キーワード |
|
|
主題 |
Sphingolipid |
| キーワード |
|
|
主題 |
Phytoceramide 1-phosphate |
| キーワード |
|
|
主題 |
Glycosylinositol phosphoceramide |
| キーワード |
|
|
主題 |
TLC |
| キーワード |
|
|
主題 |
Sephadex column chromatography |
| キーワード |
|
|
主題 |
Plants |
| 書誌情報 |
en : Journal of Chromatography B
巻 1152,
p. 122213,
発行日 2020-06-12
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
15700232 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11656178 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11644805 |
| 出版者 |
|
|
出版者 |
Elsevier |
| 権利情報 |
|
|
権利情報 |
© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| EID |
|
|
識別子 |
370497 |
| 言語 |
|
|
言語 |
eng |
jchromb_1152_122213.pdf (1.15 MB)
