{"created":"2024-11-22T06:40:24.380005+00:00","id":2008189,"links":{},"metadata":{"_buckets":{"deposit":"8d4bf7f4-e8b9-4cae-a79e-7869650121e1"},"_deposit":{"created_by":7,"id":"2008189","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2008189"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02008189","sets":["1713853213384:1713853295607"]},"author_link":["118","965"],"control_number":"2008189","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-08-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"1760","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"Frontiers in Microbiology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Many kinds of NAD(P)+-dependent L-amino acid dehydrogenases have been so far found and effectively used for synthesis of L-amino acids and their analogs, and for their sensing. By contrast, similar biotechnological use of D-amino acid dehydrogenase (D-AADH) has not been achieved because useful D-AADH has not been found from natural resources. Recently, using protein engineering methods, an NADP+-dependent D-AADH was created from meso-diaminopimelate dehydrogenase (meso-DAPDH). The artificially created D-AADH catalyzed the reversible NADP+-dependent oxidative deamination of D-amino acids to 2-oxo acids. The enzyme, especially thermostable one from thermophiles, was efficiently applicable to synthesis of D-branched-chain amino acids (D-BCAAs), with high yields and optical purity, and was useful for the practical synthesis of 13C- and/or 15N-labeled D-BCAAs. The enzyme also made it possible to assay D-isoleucine selectively in a mixture of isoleucine isomers. Analyses of the three-dimensional structures of meso-DAPDH and D-AADH, and designed mutations based on the information obtained made it possible to markedly enhance enzyme activity and to create D-AADH homologs with desired reactivity profiles. The methods described here may be an effective approach to artificial creation of biotechnologically useful enzymes.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Frontiers Media S.A.","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"1664302X","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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Hironaga","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"林, 順司","creatorNameLang":"ja"},{"creatorName":"ハヤシ, ジュンジ","creatorNameLang":"ja-Kana"},{"creatorName":"Hayashi, Junji","creatorNameLang":"en"}],"familyNames":[{"familyName":"林","familyNameLang":"ja"},{"familyName":"ハヤシ","familyNameLang":"ja-Kana"},{"familyName":"Hayashi","familyNameLang":"en"}],"givenNames":[{"givenName":"順司","givenNameLang":"ja"},{"givenName":"ジュンジ","givenNameLang":"ja-Kana"},{"givenName":"Junji","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"965","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"350215/profile-ja.html","nameIdentifierScheme":"徳島大学 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engineering","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"crystal structure analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Artificial Thermostable D-Amino Acid Dehydrogenase : Creation and Application","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Artificial Thermostable D-Amino Acid Dehydrogenase : Creation and Application","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-01-15"},"publish_date":"2021-01-15","publish_status":"0","recid":"2008189","relation_version_is_last":true,"title":["Artificial Thermostable D-Amino Acid Dehydrogenase : Creation and Application"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-29T03:33:45.135698+00:00"}