{"created":"2024-11-22T06:46:50.677981+00:00","id":2008259,"links":{},"metadata":{"_buckets":{"deposit":"eb766aef-f03d-4dbd-be79-d253e429ad3d"},"_deposit":{"created_by":7,"id":"2008259","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2008259"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02008259","sets":["1713853213384:1713853296295:1716267876683:1716268551628"]},"author_link":["882","349","852","709","710","781","175","1572","654","1643","287"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"ミリプラチン及び放射線はPUMAを介したアポトーシスにより肝細胞癌に相乗効果を示す","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"Chemoradiotherapy with miriplatin","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-07-14","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"1086","bibliographicPageStart":"1072","bibliographicVolumeNumber":"55","bibliographic_titles":[{"bibliographic_title":"Journal of Gastroenterology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: The prognosis for patients with unresectable advanced hepatocellular carcinoma (HCC) is poor. Miriplatin is a hydrophobic platinum compound that has a long retention time in lesions after transarterial chemoembolization (TACE). We investigated anti-tumor activity of miriplatin combined with irradiation on HCC cells, and its underlying mechanism of apoptosis. We also analyzed the effectiveness of miriplatin-TACE and radiotherapy for locally advanced HCC.\nMethods: Human HCC cell lines HepG2 and HuH-7 were treated with DPC (active form of miriplatin) and radiation, and synergy was evaluated using a combination index (CI). Apoptosis-related proteins and cell cycles were analyzed by western blotting and flowcytometry. We retrospectively analyzed treatment outcomes in 10 unresectable HCC patients with vascular/bile duct invasion treated with miriplatin-TACE and radiotherapy.\nResults: DPC or X-ray irradiation decreased cell viability dose-dependently. DPC plus irradiation decreased cell viability synergistically in both cell lines (CI<1 respectively). Cleaved PARP expression was induced much more strongly by DPC plus irradiation than by each treatment alone. Expression of p53 up-regulated modulator of apoptosis (PUMA) was significantly induced by the combination, and knockdown of PUMA with siRNA significantly decreased apoptosis in both cell lines. DPC plus irradiation caused sub-G1, G2/M, and S phase cell arrest in those cells. The combination of miriplatin-TACE and radiotherapy showed a high response rate for patients with locally advanced HCC despite small number of patients.\nConclusions: Miriplatin plus irradiation had synergistic anti-tumor activity on HCC cells through PUMA-mediated apoptosis and cell cycle arrest. This combination may possibly be effective in treating locally advanced HCC.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature","subitem_publisher_language":"en"},{"subitem_publisher":"Japanese Society of Gastroenterology","subitem_publisher_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"09441174","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"14355922","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA10988015","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA11627089","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3458号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2020-09-24"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開\n本論文は, 著者Hironori Tanakaの学位論文として提出され, 学位審査・授与の対象となっている。\nThis is a post-peer-review, pre-copyedit version of an article published in Journal of Gastroenterology.\nThe final authenticated version is available online at: https://doi.org/10.1007/s00535-020-01705-8\n学位授与者所属 : 徳島大学大学院医科学教育部（医学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲医第1465号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1007/s00535-020-01705-8"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s00535-020-01705-8","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"366786"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田中, 宏典","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"882","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡本, 耕一","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"349","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐藤, 康史","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"852","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田中, 貴大","creatorNameLang":"ja"},{"creatorName":"タナカ, タカヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanaka, Takahiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"友成, 哲","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"709","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中村, 文香","creatorNameLang":"ja"},{"creatorName":"ナカムラ, フミカ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakamura, Fumika","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"藤野, 泰輝","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"710","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"三井, 康裕","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"781","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"宮本, 弘志","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"175","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"六車, 直樹","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1572","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森田, 明典","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"654","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"生島, 仁史","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1643","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高山, 哲治","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"287","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2020-10-13"}],"displaytype":"detail","filename":"k3458_abstract_review.pdf","filesize":[{"value":"206 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2008259/files/k3458_abstract_review.pdf"},"version_id":"d864b69b-7b31-4888-9b74-75e8b7063ee6"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2021-07-14"}],"displaytype":"detail","filename":"k3458_fulltext.pdf","filesize":[{"value":"2.77 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2008259/files/k3458_fulltext.pdf"},"version_id":"6e01669c-4baf-4546-abdb-724ec8c31b99"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"hepatocellular carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"miriplatin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"radiation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"synergistic effect","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Synergistic anti-tumor activity of miriplatin and radiation through PUMA-mediated apoptosis in hepatocellular carcinoma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Synergistic anti-tumor activity of miriplatin and radiation through PUMA-mediated apoptosis in hepatocellular carcinoma","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268551628"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-10-13"},"publish_date":"2020-10-13","publish_status":"0","recid":"2008259","relation_version_is_last":true,"title":["Synergistic anti-tumor activity of miriplatin and radiation through PUMA-mediated apoptosis in hepatocellular carcinoma"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-11-22T06:47:00.878893+00:00"}