{"created":"2024-11-22T07:01:08.676903+00:00","id":2008422,"links":{},"metadata":{"_buckets":{"deposit":"8b864107-49af-4b5c-a3b7-770b8546fc61"},"_deposit":{"created_by":7,"id":"2008422","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2008422"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02008422","sets":["1713853213384:1713853295607"]},"author_link":["807","549"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-01-21","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"1460","bibliographicPageStart":"1453","bibliographicVolumeNumber":"413","bibliographic_titles":[{"bibliographic_title":"Analytical and Bioanalytical Chemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Kinetic reactions of the transphosphorylation with creatine kinase (CK) were individually investigated between creatine (Cr) and creatine phosphate (CrP) by pressure-assisted capillary electrophoresis/dynamic frontal analysis (pCE/DFA). The transphosphorylations are reversible between Cr and CrP, and reverse reactions inevitably accompany in general batch analyses. In pCE/DFA, the kinetic reaction proceeds in a separation capillary and the product is continuously resolved from the substrate zone. Therefore, the formation rate is kept constant at the substrate zone without the reverse reaction, and the product is detected as a plateau signal. This study demonstrates the direct and individual analyses of both the forward and the backward kinetic reactions with CK by pCE/DFA. A plateau signal was detected in the pCE/DFA with ADP or ATP as one of the products on either the forward or the backward reactions. The Michaelis-Menten constants of Km,ATP (from Cr to CrP) and Km,ADP (from CrP to Cr) were successfully determined through the plateau signal. Determined values of Km,ATP and Km,ADP by pCE/DFA were smaller than the ones obtained by the pre-capillary batch analyses. The results agree with the fact that the reverse reaction is excluded in the analysis of the kinetic reactions. The proposed pCE/DFA is useful on individual analyses of both forward and backward kinetic reactions without any interference from the reverse reaction.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature","subitem_publisher_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"16182642","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"16182650","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11610734","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"This is a post-peer-review, pre-copyedit version of an article published in Analytical and Bioanalytical Chemistry. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00216-020-03110-9."}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1007/s00216-020-03110-9"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s00216-020-03110-9","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"372562"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ミネ, マサノリ","creatorNameLang":"ja"},{"creatorName":"ミネ, マサノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Mine, Masanori","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"水口, 仁志","creatorNameLang":"ja"},{"creatorName":"ミズグチ, ヒトシ","creatorNameLang":"ja-Kana"},{"creatorName":"Mizuguchi, Hitoshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"水口","familyNameLang":"ja"},{"familyName":"ミズグチ","familyNameLang":"ja-Kana"},{"familyName":"Mizuguchi","familyNameLang":"en"}],"givenNames":[{"givenName":"仁志","givenNameLang":"ja"},{"givenName":"ヒトシ","givenNameLang":"ja-Kana"},{"givenName":"Hitoshi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"807","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"303129/profile-ja.html","nameIdentifierScheme":"徳島大学 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Toshio","creatorNameLang":"en"}],"familyNames":[{"familyName":"髙栁","familyNameLang":"ja"},{"familyName":"タカヤナギ","familyNameLang":"ja-Kana"},{"familyName":"Takayanagi","familyNameLang":"en"}],"givenNames":[{"givenName":"俊夫","givenNameLang":"ja"},{"givenName":"トシオ","givenNameLang":"ja-Kana"},{"givenName":"Toshio","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"549","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"241977/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/241977/profile-ja.html"},{"nameIdentifier":"50263554","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=50263554"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-01-21"}],"displaytype":"detail","filename":"abc_413_5_1453.pdf","filesize":[{"value":"1020 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2008422/files/abc_413_5_1453.pdf"},"version_id":"a65e9992-8581-4d37-84c1-ec168a0aed3c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Capillary electrophoresis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Dynamic frontal analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Creatine kinase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Transphosphorylation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Kinetic analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Kinetic analysis of the transphosphorylation with creatine kinase by pressure-assisted capillary electrophoresis/dynamic frontal analysis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Kinetic analysis of the transphosphorylation with creatine kinase by pressure-assisted capillary electrophoresis/dynamic frontal analysis","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-02-01"},"publish_date":"2021-02-01","publish_status":"0","recid":"2008422","relation_version_is_last":true,"title":["Kinetic analysis of the transphosphorylation with creatine kinase by pressure-assisted capillary electrophoresis/dynamic frontal analysis"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-29T13:58:06.818527+00:00"}