{"created":"2024-11-22T07:01:47.370987+00:00","id":2008430,"links":{},"metadata":{"_buckets":{"deposit":"f5db6be5-aaa9-4e61-9e80-5c5a0bc2b259"},"_deposit":{"created_by":7,"id":"2008430","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2008430"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02008430","sets":["1713853213384:1713853295607"]},"author_link":["851"],"control_number":"2008430","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Effect of SHED-CM on DPN","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-05-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"38","bibliographicPageStart":"28","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"Journal of Diabetes Investigation","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aims/Introduction: Transplantation of stem cells promotes axonal regeneration and angiogenesis in a paracrine manner. In the present study, we examined whether the secreted factors in conditioned medium of stem cells from human exfoliated deciduous teeth (SHED‐CM) had beneficial effects on diabetic polyneuropathy in mice.\nMaterials and Methods: Conditioned medium of stem cells from human exfoliated deciduous teeth was collected 48 h after culturing in serum‐free Dulbecco's modified Eagle's medium (DMEM), and separated into four fractions according to molecular weight. Dorsal root ganglion neurons from C57BL/6J mice were cultured with SHED‐CM or DMEM to evaluate the effect on neurite outgrowth. Streptozotocin‐induced diabetic mice were injected with 100 μL of SHED‐CM or DMEM into the unilateral hindlimb muscles twice a week over a period of 4 weeks. Peripheral nerve functions were evaluated by the plantar test, and motor and sensory nerve conduction velocities. Intraepidermal nerve fiber densities, capillary number‐to‐muscle fiber ratio, capillary blood flow and morphometry of sural nerves were also evaluated.\nResults: Conditioned medium of stem cells from human exfoliated deciduous teeth significantly promoted neurite outgrowth of dorsal root ganglion neurons compared with DMEM. Among four fractions of SHED‐CM, the only fraction of <6 kDa promoted the neurite outgrowth of dorsal root ganglion neurons. In addition, SHED‐CM significantly prevented decline in sensory nerve conduction velocities compared with DMEM in diabetic mice. Although SHED‐CM did not improve intraepidermal nerve fiber densities or morphometry of sural nerves, SHED‐CM ameliorated the capillary number‐to‐muscle fiber ratio and capillary blood flow.\nConclusions: These results suggested that SHED‐CM might have a therapeutic effect on diabetic polyneuropathy through promoting neurite outgrowth, and the increase in capillaries might contribute to the improvement of neural function.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Asian Association for the Study of Diabetes","subitem_publisher_language":"en"},{"subitem_publisher":"John Wiley & Sons","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"20401124","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA12488319","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1111/jdi.13085"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/jdi.13085","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"355839"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ミウラ ユラ, エミリ","creatorNameLang":"ja"},{"creatorName":"ミウラ ユラ, エミリ","creatorNameLang":"ja-Kana"},{"creatorName":"Miura‐Yura, Emiri","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ツネカワ, シン","creatorNameLang":"ja"},{"creatorName":"ツネカワ, シン","creatorNameLang":"ja-Kana"},{"creatorName":"Tsunekawa, Shin","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ナルセ, ケイコ","creatorNameLang":"ja"},{"creatorName":"ナルセ, ケイコ","creatorNameLang":"ja-Kana"},{"creatorName":"Naruse, Keiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ナカムラ, ノブヒサ","creatorNameLang":"ja"},{"creatorName":"ナカムラ, ノブヒサ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakamura, Nobuhisa","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"モテギ, ミキオ","creatorNameLang":"ja"},{"creatorName":"モテギ, ミキオ","creatorNameLang":"ja-Kana"},{"creatorName":"Motegi, Mikio","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ナカイ シモダ, ヒロミ","creatorNameLang":"ja"},{"creatorName":"ナカイ シモダ, ヒロミ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakai‐Shimoda, Hiromi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"アサノ, サエコ","creatorNameLang":"ja"},{"creatorName":"アサノ, サエコ","creatorNameLang":"ja-Kana"},{"creatorName":"Asano, Saeko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"カトウ, マコト","creatorNameLang":"ja"},{"creatorName":"カトウ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"Kato, Makoto","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヤマダ, ユウイチロウ","creatorNameLang":"ja"},{"creatorName":"ヤマダ, ユウイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamada, Yuichiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"イズモト アキタ, タカコ","creatorNameLang":"ja"},{"creatorName":"イズモト アキタ, タカコ","creatorNameLang":"ja-Kana"},{"creatorName":"Izumoto‐Akita, Takako","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"山本, 朗仁","creatorNameLang":"ja"},{"creatorName":"ヤマモト, アキヒト","creatorNameLang":"ja-Kana"},{"creatorName":"Yamamoto, Akihito","creatorNameLang":"en"}],"familyNames":[{"familyName":"山本","familyNameLang":"ja"},{"familyName":"ヤマモト","familyNameLang":"ja-Kana"},{"familyName":"Yamamoto","familyNameLang":"en"}],"givenNames":[{"givenName":"朗仁","givenNameLang":"ja"},{"givenName":"アキヒト","givenNameLang":"ja-Kana"},{"givenName":"Akihito","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"851","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"321104/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/321104/profile-ja.html"}]},{"creatorNames":[{"creatorName":"ヒメノ, タツヒト","creatorNameLang":"ja"},{"creatorName":"ヒメノ, タツヒト","creatorNameLang":"ja-Kana"},{"creatorName":"Himeno, Tatsuhito","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"コンドウ, マサキ","creatorNameLang":"ja"},{"creatorName":"コンドウ, マサキ","creatorNameLang":"ja-Kana"},{"creatorName":"Kondo, Masaki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"カトウ, ヨシロウ","creatorNameLang":"ja"},{"creatorName":"カトウ, ヨシロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Kato, Yoshiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ナカムラ, ジロウ","creatorNameLang":"ja"},{"creatorName":"ナカムラ, ジロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakamura, Jiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"カミヤ, ヒデキ","creatorNameLang":"ja"},{"creatorName":"カミヤ, ヒデキ","creatorNameLang":"ja-Kana"},{"creatorName":"Kamiya, Hideki","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2021-03-02"}],"displaytype":"detail","filename":"jdi_11_1_28.pdf","filesize":[{"value":"1.65 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2008430/files/jdi_11_1_28.pdf"},"version_id":"b8141c2d-eb16-4ec3-9928-421af9613368"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Conditioned medium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Diabetic polyneuropathy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Stem cells from human exfoliated deciduous teeth","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Secreted factors from cultured dental pulp stem cells promoted neurite outgrowth of dorsal root ganglion neurons and ameliorated neural functions in streptozotocin‐induced diabetic mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Secreted factors from cultured dental pulp stem cells promoted neurite outgrowth of dorsal root ganglion neurons and ameliorated neural functions in streptozotocin‐induced diabetic mice","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-03-02"},"publish_date":"2021-03-02","publish_status":"0","recid":"2008430","relation_version_is_last":true,"title":["Secreted factors from cultured dental pulp stem cells promoted neurite outgrowth of dorsal root ganglion neurons and ameliorated neural functions in streptozotocin‐induced diabetic mice"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-29T12:21:49.362927+00:00"}