{"created":"2024-11-22T07:01:51.881607+00:00","id":2008431,"links":{},"metadata":{"_buckets":{"deposit":"96836268-8e78-4294-8ea5-044d181e5cfc"},"_deposit":{"created_by":7,"id":"2008431","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2008431"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02008431","sets":["1713853213384:1713853295607"]},"author_link":["567"],"control_number":"2008431","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Characterization of α-gustducin","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-01-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"822","bibliographicPageStart":"814","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"Journal of Diabetes Investigation","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aims/Introduction: Taste receptors, T1rs and T2rs, and the taste‐selective G‐protein, α‐gustducin, are expressed outside the taste‐sensing system, such as enteroendocrine L cells. Here, we examined whether α‐gustducin also affects nutrition sensing and insulin secretion by pancreatic β‐cells.\nMaterials and Methods: The expression of α‐gustducin and taste receptors was evaluated in β‐cell lines, and in rat and mouse islets either by quantitative polymerase chain reaction or fluorescence immunostaining. The effects of α‐gustducin knockdown on insulin secretion and on cyclic adenosine monophosphate and intracellular Ca2+ levels in rat INS‐1 cells were estimated. Sucralose (taste receptor agonist)‐induced insulin secretion was investigated in INS‐1 cells with α‐gustducin suppression and in islets from mouse disease models.\nResults: The expression of Tas1r3 and α‐gustducin was confirmed in β‐cell lines and pancreatic islets. Basal levels of cyclic adenosine monophosphate, intracellular calcium and insulin secretion were significantly enhanced with α‐gustducin knockdown in INS‐1 cells. The expression of α‐gustducin was decreased in high‐fat diet‐fed mice and in diabetic db/db mice. Sucralose‐induced insulin secretion was not attenuated in INS‐1 cells with α‐gustducin knockdown or in mouse islets with decreased expression of α‐gustducin.\nConclusions: α‐Gustducin is involved in the regulation of cyclic adenosine monophosphate, intracellular calcium levels and insulin secretion in pancreatic β‐cells in a manner independent of taste receptor signaling. α‐Gustducin might play a novel role in β‐cell physiology and the development of type 2 diabetes.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Asian Association for the Study of Diabetes","subitem_publisher_language":"en"},{"subitem_publisher":"John Wiley & Sons","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"20401124","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA12488319","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1111/jdi.13214"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/jdi.13214","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"364049"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ウダガワ, ハルヒデ","creatorNameLang":"ja"},{"creatorName":"ウダガワ, ハルヒデ","creatorNameLang":"ja-Kana"},{"creatorName":"Udagawa, Haruhide","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヒラモト, マサキ","creatorNameLang":"ja"},{"creatorName":"ヒラモト, マサキ","creatorNameLang":"ja-Kana"},{"creatorName":"Hiramoto, Masaki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"カワグチ, ミホ","creatorNameLang":"ja"},{"creatorName":"カワグチ, ミホ","creatorNameLang":"ja-Kana"},{"creatorName":"Kawaguchi, Miho","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"上番増, 喬","creatorNameLang":"ja"},{"creatorName":"ウエバンソウ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"Uebansou, Takashi","creatorNameLang":"en"}],"familyNames":[{"familyName":"上番増","familyNameLang":"ja"},{"familyName":"ウエバンソウ","familyNameLang":"ja-Kana"},{"familyName":"Uebansou","familyNameLang":"en"}],"givenNames":[{"givenName":"喬","givenNameLang":"ja"},{"givenName":"タカシ","givenNameLang":"ja-Kana"},{"givenName":"Takashi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"567","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"246749/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/246749/profile-ja.html"},{"nameIdentifier":"10581829","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=10581829"}]},{"creatorNames":[{"creatorName":"オハラ イマイズミ, ミカ","creatorNameLang":"ja"},{"creatorName":"オハラ イマイズミ, ミカ","creatorNameLang":"ja-Kana"},{"creatorName":"Ohara‐Imaizumi, Mica","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ナンモ, タカオ","creatorNameLang":"ja"},{"creatorName":"ナンモ, タカオ","creatorNameLang":"ja-Kana"},{"creatorName":"Nammo, Takao","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ニシムラ, ワタル","creatorNameLang":"ja"},{"creatorName":"ニシムラ, ワタル","creatorNameLang":"ja-Kana"},{"creatorName":"Nishimura, Wataru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヤスダ, カズキ","creatorNameLang":"ja"},{"creatorName":"ヤスダ, カズキ","creatorNameLang":"ja-Kana"},{"creatorName":"Yasuda, Kazuki","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2021-03-02"}],"displaytype":"detail","filename":"jdi_11_4_814.pdf","filesize":[{"value":"1.64 MB"}],"format":"application/pdf","licensetype":"license_3","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2008431/files/jdi_11_4_814.pdf"},"version_id":"7d35ce7c-51b9-45b0-ad69-8d1950c3cb35"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"α-Gustducin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Insulin secretion","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Taste receptor","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of the taste receptor‐related G‐protein, α‐gustducin, in pancreatic β‐cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of the taste receptor‐related G‐protein, α‐gustducin, in pancreatic β‐cells","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-03-02"},"publish_date":"2021-03-02","publish_status":"0","recid":"2008431","relation_version_is_last":true,"title":["Characterization of the taste receptor‐related G‐protein, α‐gustducin, in pancreatic β‐cells"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-29T10:03:46.354399+00:00"}