{"created":"2024-11-22T07:12:19.348007+00:00","id":2008438,"links":{},"metadata":{"_buckets":{"deposit":"acf19b8c-1240-4fb6-ab97-121e4a2ca13b"},"_deposit":{"created_by":7,"id":"2008438","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2008438"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02008438","sets":["1713853213384:1713853295607"]},"author_link":["664"],"control_number":"2008438","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"TALE-mediated plant genome visualization","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-10-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"21","bibliographicPageEnd":"6110","bibliographicPageStart":"6101","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"Journal of Experimental Botany","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Live imaging of the dynamics of nuclear organization provides the opportunity to uncover the mechanisms responsible for four-dimensional genome architecture. Here, we describe the use of fluorescent protein (FP) fusions of transcription activator-like effectors (TALEs) to visualize endogenous genomic sequences in Arabidopsis thaliana. The ability to engineer sequence-specific TALEs permits the investigation of precise genomic sequences. We could detect TALE-FP signals associated with centromeric, telomeric, and rDNA repeats and the signal distribution was consistent with that observed by fluorescent in situ hybridization. TALE-FPs are advantageous because they permit the observation of intact tissues. We used our TALE-FP method to investigate the nuclei of several multicellular plant tissues including roots, hypocotyls, leaves, and flowers. Because TALE-FPs permit live-cell imaging, we successfully observed the temporal dynamics of centromeres and telomeres in plant organs. Fusing TALEs to multimeric FPs enhanced the signal intensity when observing telomeres. We found that the mobility of telomeres was different in subnuclear regions. Transgenic plants stably expressing TALE-FPs will provide new insights into chromatin organization and dynamics in multicellular organisms.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Society for Experimental Biology","subitem_publisher_language":"en"},{"subitem_publisher":"Oxford University Press","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"00220957","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"14602431","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00697526","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA12096046","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1093/jxb/erw371"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1093/jxb/erw371","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"347301"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"フジモト, サトル","creatorNameLang":"ja"},{"creatorName":"フジモト, サトル","creatorNameLang":"ja-Kana"},{"creatorName":"Fujimoto, Satoru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"スガノ, シゲオ","creatorNameLang":"ja"},{"creatorName":"スガノ, シゲオ","creatorNameLang":"ja-Kana"},{"creatorName":"Sugano, Shigeo S.","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"クワタ, ケイコ","creatorNameLang":"ja"},{"creatorName":"クワタ, ケイコ","creatorNameLang":"ja-Kana"},{"creatorName":"Kuwata, Keiko","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"刑部, 敬史","creatorNameLang":"ja"},{"creatorName":"オサカベ, ケイシ","creatorNameLang":"ja-Kana"},{"creatorName":"Osakabe, Keishi","creatorNameLang":"en"}],"familyNames":[{"familyName":"刑部","familyNameLang":"ja"},{"familyName":"オサカベ","familyNameLang":"ja-Kana"},{"familyName":"Osakabe","familyNameLang":"en"}],"givenNames":[{"givenName":"敬史","givenNameLang":"ja"},{"givenName":"ケイシ","givenNameLang":"ja-Kana"},{"givenName":"Keishi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"664","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"272329/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/272329/profile-ja.html"},{"nameIdentifier":"70450335","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=70450335"}]},{"creatorNames":[{"creatorName":"マツナガ, サチヒロ","creatorNameLang":"ja"},{"creatorName":"マツナガ, サチヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Matsunaga, Sachihiro","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2021-02-16"}],"displaytype":"detail","filename":"jxb_67_21_6101.pdf","filesize":[{"value":"11.1 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2008438/files/jxb_67_21_6101.pdf"},"version_id":"2432bfe4-f91a-4997-b9d0-00b6a01087a9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Centromere","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"chromatin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fluorescent protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"genome editing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"live cell imaging","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rDNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"telomere","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"transcription activator-like effector","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Visualization of specific repetitive genomic sequences with fluorescent TALEs in Arabidopsis thaliana","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Visualization of specific repetitive genomic sequences with fluorescent TALEs in Arabidopsis thaliana","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-02-16"},"publish_date":"2021-02-16","publish_status":"0","recid":"2008438","relation_version_is_last":true,"title":["Visualization of specific repetitive genomic sequences with fluorescent TALEs in Arabidopsis thaliana"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-28T02:54:35.878888+00:00"}