{"created":"2024-11-22T07:17:48.444856+00:00","id":2008493,"links":{},"metadata":{"_buckets":{"deposit":"d5d768a4-82ca-4b34-80e5-1a607d210a27"},"_deposit":{"created_by":7,"id":"2008493","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2008493"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02008493","sets":["1713853213384:1713853295607"]},"author_link":["805"],"control_number":"2008493","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-10-01","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5725340","bibliographicVolumeNumber":"2017","bibliographic_titles":[{"bibliographic_title":"Journal of Spectroscopy","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In this study, we investigated photo-induced damage to living cells during single- and multifocus excitations for coherent anti-Stokes Raman scattering (CARS) imaging. A near-infrared pulsed laser (709 nm) was used to induce cell damage. We compared the photo-induced cell damage in the single- and the multifocus excitation schemes with the condition to obtain the same CARS signal in the same frame rate. For the evaluation of cell viability, we employed 4',6-diamidino-2-phenylindole (DAPI) fluorophores that predominantly stained the damaged cells. One- and two-photon fluorescence of DAPI fluorophores were, respectively, excited by an ultraviolet light source and the same near-infrared light source and were monitored to evaluate the cell viability during near-infrared pulsed laser irradiation. We found lower uptake of DAPI fluorophores into HeLa cells during the multifocus excitation compared with the single-focus excitation scheme in both the one- and the two-photon fluorescence examinations. This indicates a reduction of photo-induced cell damage in the multifocus excitation. Our findings suggested that the multifocus excitation scheme is expected to be suitable for CARS microscopy in terms of minimal invasiveness.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Hindawi","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2017 Takeo Minamikawa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"23144920","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"23144939","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA12661402","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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ナオカズ","creatorNameLang":"ja-Kana"},{"creatorName":"Matsumura, Naokazu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ニイオカ, ヒロヒコ","creatorNameLang":"ja"},{"creatorName":"ニイオカ, ヒロヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Niioka, Hirohiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"フクシマ, シュウイチロウ","creatorNameLang":"ja"},{"creatorName":"フクシマ, シュウイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Fukushima, Shuichiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"荒木, 勉","creatorNameLang":"ja"},{"creatorName":"アラキ, ツトム","creatorNameLang":"ja-Kana"},{"creatorName":"Araki, Tsutomu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ハシモト, マモル","creatorNameLang":"ja"},{"creatorName":"ハシモト, マモル","creatorNameLang":"ja-Kana"},{"creatorName":"Hashimoto, 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Microscopy","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Photo-Induced Cell Damage Analysis for Single- and Multifocus Coherent Anti-Stokes Raman Scattering Microscopy","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-02-19"},"publish_date":"2021-02-19","publish_status":"0","recid":"2008493","relation_version_is_last":true,"title":["Photo-Induced Cell Damage Analysis for Single- and Multifocus Coherent Anti-Stokes Raman Scattering Microscopy"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-04-28T06:02:45.528487+00:00"}