{"created":"2024-11-22T07:28:56.637388+00:00","id":2008613,"links":{},"metadata":{"_buckets":{"deposit":"3b940ae5-8bc6-435d-9d71-498b980ebcb6"},"_deposit":{"created_by":7,"id":"2008613","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2008613"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02008613","sets":["1713853213384:1713853295607"]},"author_link":["842"],"control_number":"2008613","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-05-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageStart":"577","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"Plants","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Phenylpropanoid volatile components in plants are useful and valuable not only as flavorings, but also as medicines and food supplements. The pharmacological actions and toxicities of these compounds have been well studied but their synthetic pathways are generally unclear. In this study, we mined expressed sequence tag libraries of pure strains of perilla maintained for over 30 years for their oil type and conducted gas chromatography-mass spectrometry analyses of the perilla oils to confirm the presence of monohydrates speculated to be intermediates of the phenylpropene synthetics pathways. These putative monohydrate intermediates and their regioisomers were synthesized to identify the reaction products of assays of heterologously expressed enzymes. An enzyme involved in the synthesis of a phenylpropanoid volatile component was identified in perilla. Expression of this enzyme in Saccharomyces cerevisiae showed that it is a member of the cytochrome P450 family and catalyzes the introduction of a hydroxy group onto myristicin to form an intermediate of dillapiole. The enzyme had high sequence similarity to a CYP71D family enzyme, high regiospecificity, and low substrate specificity. This study may aid the elucidation of generally unexploited biosynthetic pathways of phenylpropanoid volatile components.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"22237747","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.3390/plants9050577"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/plants9050577","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"365689"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ババ, マリコ","creatorNameLang":"ja"},{"creatorName":"ババ, マリコ","creatorNameLang":"ja-Kana"},{"creatorName":"Baba, Mariko","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"山田, 健一","creatorNameLang":"ja"},{"creatorName":"ヤマダ, ケンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamada, Ken-ichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"山田","familyNameLang":"ja"},{"familyName":"ヤマダ","familyNameLang":"ja-Kana"},{"familyName":"Yamada","familyNameLang":"en"}],"givenNames":[{"givenName":"健一","givenNameLang":"ja"},{"givenName":"ケンイチ","givenNameLang":"ja-Kana"},{"givenName":"Ken-ichi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"842","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"314544/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/314544/profile-ja.html"}]},{"creatorNames":[{"creatorName":"イトウ, ミチホ","creatorNameLang":"ja"},{"creatorName":"イトウ, ミチホ","creatorNameLang":"ja-Kana"},{"creatorName":"Ito, Michiho","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2021-04-23"}],"displaytype":"detail","filename":"plants_9_5_577.pdf","filesize":[{"value":"5.34 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2008613/files/plants_9_5_577.pdf"},"version_id":"a31a52af-364a-433d-ba77-d14a0763ae38"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"phenylpropanoid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"volatile compound","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"molecular cloning","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cytochrome P450","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"biosynthetic pathway","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"perilla","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cloning and Expression of a Perilla frutescens Cytochrome P450 Enzyme Catalyzing the Hydroxylation of Phenylpropenes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cloning and Expression of a Perilla frutescens Cytochrome P450 Enzyme Catalyzing the Hydroxylation of Phenylpropenes","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-04-23"},"publish_date":"2021-04-23","publish_status":"0","recid":"2008613","relation_version_is_last":true,"title":["Cloning and Expression of a Perilla frutescens Cytochrome P450 Enzyme Catalyzing the Hydroxylation of Phenylpropenes"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-02-21T00:51:18.868660+00:00"}