{"created":"2024-11-22T07:45:03.086135+00:00","id":2008803,"links":{},"metadata":{"_buckets":{"deposit":"9785480c-6f93-4421-b5a3-bf2524f3f573"},"_deposit":{"created_by":7,"id":"2008803","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2008803"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02008803","sets":["1713853213384:1713853295607"]},"author_link":["560"],"control_number":"2008803","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-10-26","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageStart":"1482","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"Biomolecules","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Baculovirus-infected silkworms are promising bioreactors for producing recombinant glycoproteins, including antibodies. Previously, we developed a method for isotope labeling of glycoproteins for nuclear magnetic resonance (NMR) studies using silkworm larvae reared on an artificial diet containing 15N-labeled yeast crude protein extract. Here, we further develop this method by introducing a technique for the expression of isotope-labeled glycoproteins by silkworm pupae, which has several potential advantages relative to larvae-based techniques in terms of production yield, ease of handling, and storage. Here, we fed fifth instar larvae an artificial diet with an optimized composition containing [methyl-13C]methionine, leading to pupation. Nine-day-old pupae were then injected with recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid for expression of recombinant human immunoglobulin G (IgG). From the whole-body homogenates of pupae, 0.35 mg/pupa of IgG was harvested, which is a yield that is five times higher than can be obtained from larvae. Recombinant IgG, thus prepared, exhibited mainly three kinds of pauci-mannose-type oligosaccharides and had a 13C-enrichment ratio of approximately 80%. This enabled selective observation of NMR signals originating from the methionyl methyl group of IgG, confirming its conformational integrity. These data demonstrate the utility of silkworm pupae as factories for producing recombinant glycoproteins with amino-acid-selective isotope labeling.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"2218273X","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.3390/biom10111482"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/biom10111482","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"373149"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ヤギ, ヒロカズ","creatorNameLang":"ja"},{"creatorName":"ヤギ, ヒロカズ","creatorNameLang":"ja-Kana"},{"creatorName":"Yagi, Hirokazu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヤナカ, サエコ","creatorNameLang":"ja"},{"creatorName":"ヤナカ, サエコ","creatorNameLang":"ja-Kana"},{"creatorName":"Yanaka, Saeko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヨゴ, リナ","creatorNameLang":"ja"},{"creatorName":"ヨゴ, リナ","creatorNameLang":"ja-Kana"},{"creatorName":"Yogo, Rina","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"イケダ, アカリ","creatorNameLang":"ja"},{"creatorName":"イケダ, アカリ","creatorNameLang":"ja-Kana"},{"creatorName":"Ikeda, Akari","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"鬼塚, 正義","creatorNameLang":"ja"},{"creatorName":"オニツカ, マサヨシ","creatorNameLang":"ja-Kana"},{"creatorName":"Onitsuka, Masayoshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"鬼塚","familyNameLang":"ja"},{"familyName":"オニツカ","familyNameLang":"ja-Kana"},{"familyName":"Onitsuka","familyNameLang":"en"}],"givenNames":[{"givenName":"正義","givenNameLang":"ja"},{"givenName":"マサヨシ","givenNameLang":"ja-Kana"},{"givenName":"Masayoshi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"560","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"246724/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/246724/profile-ja.html"},{"nameIdentifier":"80571174","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=80571174"}]},{"creatorNames":[{"creatorName":"ヤマザキ, トシオ","creatorNameLang":"ja"},{"creatorName":"ヤマザキ, トシオ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamazaki, Toshio","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"カトウ, タツヤ","creatorNameLang":"ja"},{"creatorName":"カトウ, タツヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Kato, Tatsuya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Park, Enoch Y.","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヨコヤマ, ジュン","creatorNameLang":"ja"},{"creatorName":"ヨコヤマ, ジュン","creatorNameLang":"ja-Kana"},{"creatorName":"Yokoyama, Jun","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"カトウ, コウイチ","creatorNameLang":"ja"},{"creatorName":"カトウ, コウイチ","creatorNameLang":"ja-Kana"},{"creatorName":"Kato, Koichi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2021-06-11"}],"displaytype":"detail","filename":"biom_10_11_1482.pdf","filesize":[{"value":"2.32 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2008803/files/biom_10_11_1482.pdf"},"version_id":"6e4b09e8-800a-47a2-acf4-6611c4d258cc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"silkworm pupa","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"isotope labeling","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"recombinant glycoprotein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"artificial diet","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"immunoglobulin G","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Silkworm Pupae Function as Efficient Producers of Recombinant Glycoproteins with Stable-Isotope Labeling","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Silkworm Pupae Function as Efficient Producers of Recombinant Glycoproteins with Stable-Isotope Labeling","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-06-11"},"publish_date":"2021-06-11","publish_status":"0","recid":"2008803","relation_version_is_last":true,"title":["Silkworm Pupae Function as Efficient Producers of Recombinant Glycoproteins with Stable-Isotope Labeling"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-29T09:54:19.230976+00:00"}