{"created":"2024-11-22T07:45:31.966274+00:00","id":2008809,"links":{},"metadata":{"_buckets":{"deposit":"1c5e9d9a-f66b-4d04-b924-50ae047b39b8"},"_deposit":{"created_by":7,"id":"2008809","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2008809"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02008809","sets":["1713853213384:1713853295607"]},"author_link":["748","664","902"],"control_number":"2008809","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-11-06","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"648","bibliographicVolumeNumber":"3","bibliographic_titles":[{"bibliographic_title":"Communications Biology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Genome editing in plants has advanced greatly by applying the clustered regularly interspaced short palindromic repeats (CRISPRs)-Cas system, especially CRISPR-Cas9. However, CRISPR type I—the most abundant CRISPR system in bacteria—has not been exploited for plant genome modification. In type I CRISPR-Cas systems, e.g., type I-E, Cas3 nucleases degrade the target DNA in mammals. Here, we present a type I-D (TiD) CRISPR-Cas genome editing system in plants. TiD lacks the Cas3 nuclease domain; instead, Cas10d is the functional nuclease in vivo. TiD was active in targeted mutagenesis of tomato genomic DNA. The mutations generated by TiD differed from those of CRISPR/Cas9; both bi-directional long-range deletions and short indels mutations were detected in tomato cells. Furthermore, TiD can be used to efficiently generate bi-allelic mutant plants in the first generation. These findings indicate that TiD is a unique CRISPR system that can be used for genome engineering in plants.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"23993642","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1038/s42003-020-01366-6"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/s42003-020-01366-6","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"372338"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"刑部, 敬史","creatorNameLang":"ja"},{"creatorName":"オサカベ, ケイシ","creatorNameLang":"ja-Kana"},{"creatorName":"Osakabe, Keishi","creatorNameLang":"en"}],"familyNames":[{"familyName":"刑部","familyNameLang":"ja"},{"familyName":"オサカベ","familyNameLang":"ja-Kana"},{"familyName":"Osakabe","familyNameLang":"en"}],"givenNames":[{"givenName":"敬史","givenNameLang":"ja"},{"givenName":"ケイシ","givenNameLang":"ja-Kana"},{"givenName":"Keishi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"664","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"272329/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/272329/profile-ja.html"},{"nameIdentifier":"70450335","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=70450335"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"和田, 直樹","creatorNameLang":"ja"},{"creatorName":"ワダ, ナオキ","creatorNameLang":"ja-Kana"},{"creatorName":"Wada, Naoki","creatorNameLang":"en"}],"familyNames":[{"familyName":"和田","familyNameLang":"ja"},{"familyName":"ワダ","familyNameLang":"ja-Kana"},{"familyName":"Wada","familyNameLang":"en"}],"givenNames":[{"givenName":"直樹","givenNameLang":"ja"},{"givenName":"ナオキ","givenNameLang":"ja-Kana"},{"givenName":"Naoki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"902","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"334009/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/334009/profile-ja.html"}]},{"creatorNames":[{"creatorName":"ミヤジ, トモコ","creatorNameLang":"ja"},{"creatorName":"ミヤジ, トモコ","creatorNameLang":"ja-Kana"},{"creatorName":"Miyaji, Tomoko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ムラカミ, エミ","creatorNameLang":"ja"},{"creatorName":"ムラカミ, エミ","creatorNameLang":"ja-Kana"},{"creatorName":"Murakami, Emi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"マルイ, カズヤ","creatorNameLang":"ja"},{"creatorName":"マルイ, カズヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Marui, Kazuya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ウエタ, リサ","creatorNameLang":"ja"},{"creatorName":"ウエタ, リサ","creatorNameLang":"ja-Kana"},{"creatorName":"Ueta, Risa","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ハシモト, リョウスケ","creatorNameLang":"ja"},{"creatorName":"ハシモト, リョウスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Hashimoto, Ryosuke","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"アベ ハラ, チヒロ","creatorNameLang":"ja"},{"creatorName":"アベ ハラ, チヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Abe-Hara, Chihiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kong, Bihe","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヤノ, ケンタロウ","creatorNameLang":"ja"},{"creatorName":"ヤノ, ケンタロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Yano, Kentaro","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"刑部, 祐里子","creatorNameLang":"ja"},{"creatorName":"オサカベ, ユリコ","creatorNameLang":"ja-Kana"},{"creatorName":"Osakabe, Yuriko","creatorNameLang":"en"}],"familyNames":[{"familyName":"刑部","familyNameLang":"ja"},{"familyName":"オサカベ","familyNameLang":"ja-Kana"},{"familyName":"Osakabe","familyNameLang":"en"}],"givenNames":[{"givenName":"祐里子","givenNameLang":"ja"},{"givenName":"ユリコ","givenNameLang":"ja-Kana"},{"givenName":"Yuriko","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"748","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50444071","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=50444071"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2021-06-24"}],"displaytype":"detail","filename":"cb_3_648.pdf","filesize":[{"value":"3.05 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2008809/files/cb_3_648.pdf"},"version_id":"b85d8e26-a778-4074-bb9d-2e080769c25d"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Genome editing in plants using CRISPR type I-D nuclease","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Genome editing in plants using CRISPR type I-D nuclease","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-06-24"},"publish_date":"2021-06-24","publish_status":"0","recid":"2008809","relation_version_is_last":true,"title":["Genome editing in plants using CRISPR type I-D nuclease"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-02-13T06:36:07.211038+00:00"}