{"created":"2024-12-12T01:41:21.350058+00:00","id":2009434,"links":{},"metadata":{"_buckets":{"deposit":"dc0b1403-a6de-4082-966f-6c871a8142d0"},"_deposit":{"created_by":7,"id":"2009434","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2009434"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02009434","sets":["1713853213384:1713853295607"]},"author_link":["614"],"control_number":"2009434","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-05-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"1764","bibliographicPageStart":"1757","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"Infection and Drug Resistance","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Helicobacter pylori infection poses a risk of the occurrence of gastrointestinal diseases, such as gastric cancer. Its incidence rate is significantly reduced by eradication, and thereby, eradication therapy is generally performed. Disulfiram is an oral prescription drug mainly used for the treatment of alcohol dependence. In recent years, reports have been made on its anticancer and antibacterial effects, and thus, it has recently become an interesting subject. This study aimed to examine the antibacterial activity of disulfiram, investigate the presence or absence of its antibacterial activity on H. pylori, and determine whether it could be a new bactericidal drug against drug-resistant H. pylori.\nMaterials and Methods: Drug-sensitive strains of H. pylori and amoxicillin-resistant, clarithromycin-resistant, and metronidazole-resistant strains were used, and a growth inhibition test of H. pylori using disulfiram was performed. Furthermore, the expression of urease, vacuolating cytotoxin A (VacA), and CagA, the virulence proteins of H. pylori, was quantitatively analyzed using the Western blotting method. In addition, for H. pylori used in this study, the 16SrDNA sequence, a ribosomal gene involved in protein production, was analyzed to examine the presence or absence of gene mutation.\nResults: Disulfiram suppressed the growth of 7 out of 12 H. pylori strains at 1 μg/mL, and no correlation was observed between their susceptibility/resistance to current eradication antimicrobial drugs and disulfiram resistance. Disulfiram reduced the expression levels of urease, VacA, and CagA proteins. H. pylori, which showed resistance to disulfiram, tended to have fewer gene deletions/insertions in the 16S rDNA sequence; however, no specific mutation was detected.\nConclusion: Disulfiram has a bactericidal effect on H. pylori at low concentrations, suggesting that it can be used as a supplement for current H. pylori eradication drugs.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Dove Medical Press","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms. php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"11786973","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"所属英表記誤記あり　(誤)Koch Medical School→(正)Kochi Medical School"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.2147/IDR.S299177"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2147/IDR.S299177","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"377841"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"コバタケ, トモミ","creatorNameLang":"ja"},{"creatorName":"コバタケ, トモミ","creatorNameLang":"ja-Kana"},{"creatorName":"Kobatake, Tomomi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"オギノ, ケイキ","creatorNameLang":"ja"},{"creatorName":"オギノ, ケイキ","creatorNameLang":"ja-Kana"},{"creatorName":"Ogino, Keiki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"サカエ, ヒロユキ","creatorNameLang":"ja"},{"creatorName":"サカエ, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Sakae, Hiroyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ゴトウ, カズヨシ","creatorNameLang":"ja"},{"creatorName":"ゴトウ, カズヨシ","creatorNameLang":"ja-Kana"},{"creatorName":"Gotoh, Kazuyoshi","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"渡辺, 朱理","creatorNameLang":"ja"},{"creatorName":"ワタナベ, アカリ","creatorNameLang":"ja-Kana"},{"creatorName":"Watanabe, Akari","creatorNameLang":"en"}],"familyNames":[{"familyName":"渡辺","familyNameLang":"ja"},{"familyName":"ワタナベ","familyNameLang":"ja-Kana"},{"familyName":"Watanabe","familyNameLang":"en"}],"givenNames":[{"givenName":"朱理","givenNameLang":"ja"},{"givenName":"アカリ","givenNameLang":"ja-Kana"},{"givenName":"Akari","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"614","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"261377/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/261377/profile-ja.html"},{"nameIdentifier":"80585026","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=80585026"}]},{"creatorNames":[{"creatorName":"マツシタ, オサム","creatorNameLang":"ja"},{"creatorName":"マツシタ, オサム","creatorNameLang":"ja-Kana"},{"creatorName":"Matsushita, Osamu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"オカダ, ヒロユキ","creatorNameLang":"ja"},{"creatorName":"オカダ, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Okada, Hiroyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヨコタ, ケンジ","creatorNameLang":"ja"},{"creatorName":"ヨコタ, ケンジ","creatorNameLang":"ja-Kana"},{"creatorName":"Yokota, Kenji","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2021-12-22"}],"displaytype":"detail","filename":"idr_14_1757.pdf","filesize":[{"value":"435 KB"}],"format":"application/pdf","licensetype":"license_9","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2009434/files/idr_14_1757.pdf"},"version_id":"00dde422-7f8b-445b-9faf-6e7bde002c22"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"disulfiram","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Helicobacter pylori","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"urease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"vacuolating toxin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CagA","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Antibacterial Effects of Disulfiram in Helicobacter pylori","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Antibacterial Effects of Disulfiram in Helicobacter pylori","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-12-22"},"publish_date":"2021-12-22","publish_status":"0","recid":"2009434","relation_version_is_last":true,"title":["Antibacterial Effects of Disulfiram in Helicobacter pylori"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-04-28T05:00:52.537050+00:00"}