{"created":"2024-12-12T07:23:51.861232+00:00","id":2009876,"links":{},"metadata":{"_buckets":{"deposit":"e45ddfce-ae29-4c09-a2b0-8761097edab7"},"_deposit":{"created_by":7,"id":"2009876","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2009876"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02009876","sets":["1713853213384:1713853296295:1716267878536:1716268699858"]},"author_link":["428","864","586","365"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"CRISPR-Cas9システムを用いた味覚受容体発現調節物質のスクリーニング系の開発","subitem_alternative_title_language":"ja"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-02-23","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"72","bibliographicPageStart":"65","bibliographicVolumeNumber":"601","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Taste recognition mediated by taste receptors is critical for the survival of animals in nature and is an important determinant of nutritional status and quality of life in humans. However, many factors including aging, diabetes, zinc deficiency, infection with influenza or cold viruses, and chemotherapy can trigger dysgeusia, for which a standard treatment has not been established. We here established an engineered strain of medaka (Oryzias latipes) that expresses green fluorescent protein (GFP) from the endogenous taste 1 receptor 3 (T1R3) gene locus with the use of the CRISPR-Cas9 system. This T1R3-GFP knock-in (KI) strain allows direct visualization of expression from this locus by monitoring of GFP fluorescence. The pattern of GFP expression in the T1R3-GFP KI fish thus mimicked that of endogenous T1R3 gene expression. Furthermore, exposure of T1R3-GFP KI medaka to water containing monosodium glutamate or the anticancer agent 5-fluorouracil resulted in an increase or decrease, respectively, in GFP fluorescence intensity, effects that also recapitulated those on T1R3 mRNA abundance. Finally, screening for agents that affect GFP fluorescence intensity in T1R3-GFP KI medaka identified tryptophan as an amino acid that increases T1R3 gene expression. The establishment of this screening system for taste receptor expression in medaka provides a new tool for the development of potential therapeutic agents for dysgeusia.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"0006291X","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00564395","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA11542044","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3600号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（栄養学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-03-23"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開\n本論文は，著者Kana Beppuの学位論文として提出され，学位審査・授与の対象となっている。\n学位授与者所属 : 徳島大学大学院栄養生命科学教育部（人間栄養科学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲栄第288号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1016/j.bbrc.2022.02.082"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbrc.2022.02.082","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"388474"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"別府, 香名","creatorNameLang":"ja"},{"creatorName":"ベップ, カナ","creatorNameLang":"ja-Kana"},{"creatorName":"Beppu, Kana","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"堤, 理恵","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"428","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"アンサイ, サトシ","creatorNameLang":"ja"},{"creatorName":"アンサイ, サトシ","creatorNameLang":"ja-Kana"},{"creatorName":"Ansai, Satoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"オチアイ, ナナ","creatorNameLang":"ja"},{"creatorName":"オチアイ, ナナ","creatorNameLang":"ja-Kana"},{"creatorName":"Ochiai, Nana","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"テラカワ, マイ","creatorNameLang":"ja"},{"creatorName":"テラカワ, マイ","creatorNameLang":"ja-Kana"},{"creatorName":"Terakawa, Mai","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"モリ, マリエ","creatorNameLang":"ja"},{"creatorName":"モリ, マリエ","creatorNameLang":"ja-Kana"},{"creatorName":"Mori, Marie","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"黒田, 雅士","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"864","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"堀川, 一樹","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"586","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"トモイ, タクミ","creatorNameLang":"ja"},{"creatorName":"トモイ, タクミ","creatorNameLang":"ja-Kana"},{"creatorName":"Tomoi, Takumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"サカモト, ジョウ","creatorNameLang":"ja"},{"creatorName":"サカモト, ジョウ","creatorNameLang":"ja-Kana"},{"creatorName":"Sakamoto, Joe","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"カメイ, ヤスヒロ","creatorNameLang":"ja"},{"creatorName":"カメイ, ヤスヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Kamei, Yasuhiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ナルセ, キヨシ","creatorNameLang":"ja"},{"creatorName":"ナルセ, キヨシ","creatorNameLang":"ja-Kana"},{"creatorName":"Naruse, Kiyoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"阪上, 浩","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"365","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-05-25"}],"displaytype":"detail","filename":"k3600_abstract_review.pdf","filesize":[{"value":"179 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2009876/files/k3600_abstract_review.pdf"},"version_id":"a2d3412a-ff11-41b3-a87b-076aa699be24"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-05-25"}],"displaytype":"detail","filename":"k3600_fulltext.pdf","filesize":[{"value":"1.35 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2009876/files/k3600_fulltext.pdf"},"version_id":"a0e97fcc-9458-47d4-837a-c1d31005e518"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"taste 1 receptor 3","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"dysgeusia","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"medaka (Oryzias latipes)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CRISPR-Cas9 system","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"monosodium glutamate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"5-fluorouracil","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Development of a screening system for agents that modulate taste receptor expression with the CRISPR-Cas9 system in medaka","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of a screening system for agents that modulate taste receptor expression with the CRISPR-Cas9 system in medaka","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268699858"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-05-25"},"publish_date":"2022-05-25","publish_status":"0","recid":"2009876","relation_version_is_last":true,"title":["Development of a screening system for agents that modulate taste receptor expression with the CRISPR-Cas9 system in medaka"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-12-12T07:24:00.868644+00:00"}