{"created":"2024-12-12T07:26:28.121502+00:00","id":2009908,"links":{},"metadata":{"_buckets":{"deposit":"f8d55a88-a70c-40c6-8a37-5a1ec8a6a7dd"},"_deposit":{"created_by":7,"id":"2009908","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2009908"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02009908","sets":["1713853213384:1713853295607"]},"author_link":["1255","1156","1264","424","174","1648"],"control_number":"2009908","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-10-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"13","bibliographicPageStart":"1","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"Islets","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The aim of this study was to identify genes that are specifically expressed in pancreatic islet β-cells (hereafter referred to as β-cells). Large-scale complementary DNA-sequencing analysis was performed for 3,429 expressed sequence tags derived from murine MIN6 β-cells, through homology comparisons using the GenBank database. Three individual ESTs were found to code for protease serine S1 family member 53 (Prss53). Prss53 mRNA is processed into both a short and long form, which encode 482 and 552 amino acids, respectively. Transient overexpression of myc-tagged Prss53 in COS-7 cells showed that Prss53 was strongly associated with the luminal surfaces of organellar membranes and that it underwent signal peptide cleavage and N-glycosylation. Immunoelectron microscopy and western blotting revealed that Prss53 localized to mitochondria in MIN6 cells. Short hairpin RNA-mediated Prss53 knockdown resulted in Ppargc1a downregulation and Ucp2 and Glut2 upregulation. JC-1 staining revealed that the mitochondria were depolarized in Prss53-knockdown MIN6 cells; however, no change was observed in glucose-stimulated insulin secretion. Our results suggest that mitochondrial Prss53 expression plays an important role in maintaining the health of β-cells.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Taylor & Francis","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"19382014","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"19382022","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1080/19382014.2021.1982325"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1080/19382014.2021.1982325","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"385107"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"水澤, 典子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1255","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"原田, 永勝","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1156","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岩田, 武男","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1264","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大東, いずみ","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"424","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"板倉, 光夫","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"174","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉本, 勝彦","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1648","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-05-17"}],"displaytype":"detail","filename":"islets_14_1_1.pdf","filesize":[{"value":"1.34 MB"}],"format":"application/pdf","licensetype":"license_3","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2009908/files/islets_14_1_1.pdf"},"version_id":"e0748fa3-3047-41c8-bcad-307eb9cccf2b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Prss53","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pancreatic β-cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MIN6","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mitochondria","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of protease serine S1 family member 53 as a mitochondrial protein in murine islet beta cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of protease serine S1 family member 53 as a mitochondrial protein in murine islet beta cells","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-05-17"},"publish_date":"2022-05-17","publish_status":"0","recid":"2009908","relation_version_is_last":true,"title":["Identification of protease serine S1 family member 53 as a mitochondrial protein in murine islet beta cells"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-12-20T00:26:03.567379+00:00"}