{"created":"2024-12-12T07:33:27.891284+00:00","id":2009989,"links":{},"metadata":{"_buckets":{"deposit":"aa0f538d-f5d7-444a-816e-64e1fb3c4473"},"_deposit":{"created_by":7,"id":"2009989","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2009989"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02009989","sets":["1713853213384:1713853296295:1716267878536:1716268699858"]},"author_link":["1583","949","761","1585","1274","1507"],"control_number":"2009989","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"25-ヒドロキシビタミンＤ-1α-水酸化酵素は異所性石灰化を誘導する","subitem_alternative_title_language":"ja"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-06-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"111","bibliographicPageStart":"103","bibliographicVolumeNumber":"71","bibliographic_titles":[{"bibliographic_title":"Journal of Clinical Biochemistry and Nutrition","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients. It has been well-known that hyper­phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney. However, the detailed mechanism of the ectopic calcification has been not clarified yet. Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs. Under high phosphate condition, overexpression of CYP27B1 induced calcification and osteocalcin mRNA expression in the VSMCs. Inversely, siRNA-CYP27B1 inhibited high phosphate-induced calcification of the VSMCs. We also found that the accumulated CYP27B1 protein was glycosylated in the kidney of kl/kl mice. Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1. These results suggest that extra-renal expression of glycosylated CYP27B1 would be required for ectopic calcification of VSMCs under hyperphosphatemia.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Society for Free Radical Research Japan","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (https://creativecommons.org/licenses/by-nc-nd/4.0/).","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"09120009","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"18805086","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA10710201","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3636号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（栄養学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-03-24"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開\n本論文は，著者Yilimulati Yimamuの学位論文として提出され，学位審査・授与の対象となっている。\n学位授与者所属 : 徳島大学大学院栄養生命科学教育部（人間栄養科学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲栄第291号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.3164/jcbn.22-16"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3164/jcbn.22-16","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"398811"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"イマム, 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calcification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"arteriosclerosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"chronic kidney disease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bone and mineral disease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"dialysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"25-hydroxyvitamin D-1α-hydroxylase (CYP27B1) induces ectopic calcification","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"25-hydroxyvitamin D-1α-hydroxylase (CYP27B1) induces ectopic calcification","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268699858"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-06-06"},"publish_date":"2022-06-06","publish_status":"0","recid":"2009989","relation_version_is_last":true,"title":["25-hydroxyvitamin D-1α-hydroxylase (CYP27B1) induces ectopic calcification"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-02-14T00:26:43.892352+00:00"}