{"created":"2024-12-12T07:38:48.797324+00:00","id":2010057,"links":{},"metadata":{"_buckets":{"deposit":"ff057f0e-cd02-4bd5-9d81-acf890622bd0"},"_deposit":{"created_by":7,"id":"2010057","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2010057"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02010057","sets":["1713853213384:1713853295607"]},"author_link":["725","415"],"control_number":"2010057","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-09-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"2816","bibliographicPageStart":"2803","bibliographicVolumeNumber":"64","bibliographic_titles":[{"bibliographic_title":"Diabetologia","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aims/hypothesis\nPancreatic polypeptide (PP) cells, which secrete PP (encoded by the Ppy gene), are a minor population of pancreatic endocrine cells. Although it has been reported that the loss of beta cell identity might be associated with beta-to-PP cell-fate conversion, at present, little is known regarding the characteristics of Ppy-lineage cells.\nMethods\nWe used Ppy-Cre driver mice and a PP-specific monoclonal antibody to investigate the association between Ppy-lineage cells and beta cells. The molecular profiles of endocrine cells were investigated by single-cell transcriptome analysis and the glucose responsiveness of beta cells was assessed by Ca2+ imaging. Diabetic conditions were experimentally induced in mice by either streptozotocin or diphtheria toxin.\nResults\nPpy-lineage cells were found to contribute to the four major types of endocrine cells, including beta cells. Ppy-lineage beta cells are a minor subpopulation, accounting for 12–15% of total beta cells, and are mostly (81.2%) localised at the islet periphery. Unbiased single-cell analysis with a Ppy-lineage tracer demonstrated that beta cells are composed of seven clusters, which are categorised into two groups (i.e. Ppy-lineage and non-Ppy-lineage beta cells). These subpopulations of beta cells demonstrated distinct characteristics regarding their functionality and gene expression profiles. Ppy-lineage beta cells had a reduced glucose-stimulated Ca2+ signalling response and were increased in number in experimental diabetes models.\nConclusions/interpretation\nOur results indicate that an unexpected degree of beta cell heterogeneity is defined by Ppy gene activation, providing valuable insight into the homeostatic regulation of pancreatic islets and future therapeutic strategies against diabetes.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature","subitem_publisher_language":"en"},{"subitem_publisher":"European Association for the Study of Diabetes","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"0012186X","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"14320428","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00628104","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1007/s00125-021-05560-x"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s00125-021-05560-x","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"386005"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"フカイシ, タカヒロ","creatorNameLang":"ja"},{"creatorName":"フカイシ, タカヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Fukaishi, Takahiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ナカガワ, ユウコ","creatorNameLang":"ja"},{"creatorName":"ナカガワ, ユウコ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakagawa, Yuko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"フクナカ, アヤコ","creatorNameLang":"ja"},{"creatorName":"フクナカ, アヤコ","creatorNameLang":"ja-Kana"},{"creatorName":"Fukunaka, Ayako","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"サトウ, タカシ","creatorNameLang":"ja"},{"creatorName":"サトウ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"Sato, Takashi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ハラ, アケミ","creatorNameLang":"ja"},{"creatorName":"ハラ, アケミ","creatorNameLang":"ja-Kana"},{"creatorName":"Hara, Akemi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ナカオ, ケイコ","creatorNameLang":"ja"},{"creatorName":"ナカオ, ケイコ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakao, Keiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"サイトウ, ミチコ","creatorNameLang":"ja"},{"creatorName":"サイトウ, ミチコ","creatorNameLang":"ja-Kana"},{"creatorName":"Saito, Michiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"コウノ, ケンジ","creatorNameLang":"ja"},{"creatorName":"コウノ, ケンジ","creatorNameLang":"ja-Kana"},{"creatorName":"Kohno, Kenji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ミヤツカ, タケシ","creatorNameLang":"ja"},{"creatorName":"ミヤツカ, タケシ","creatorNameLang":"ja-Kana"},{"creatorName":"Miyatsuka, Takeshi","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"田蒔, 基行","creatorNameLang":"ja"},{"creatorName":"タマキ, モトユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Tamaki, Motoyuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"田蒔","familyNameLang":"ja"},{"familyName":"タマキ","familyNameLang":"ja-Kana"},{"familyName":"Tamaki","familyNameLang":"en"}],"givenNames":[{"givenName":"基行","givenNameLang":"ja"},{"givenName":"モトユキ","givenNameLang":"ja-Kana"},{"givenName":"Motoyuki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"725","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60624400","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=60624400"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"松久, 宗英","creatorNameLang":"ja"},{"creatorName":"マツヒサ, ムネヒデ","creatorNameLang":"ja-Kana"},{"creatorName":"Matsuhisa, Munehide","creatorNameLang":"en"}],"familyNames":[{"familyName":"松久","familyNameLang":"ja"},{"familyName":"マツヒサ","familyNameLang":"ja-Kana"},{"familyName":"Matsuhisa","familyNameLang":"en"}],"givenNames":[{"givenName":"宗英","givenNameLang":"ja"},{"givenName":"ムネヒデ","givenNameLang":"ja-Kana"},{"givenName":"Munehide","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"415","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"205776/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/205776/profile-ja.html"},{"nameIdentifier":"60362737","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=60362737"}]},{"creatorNames":[{"creatorName":"マツオカ, タカアキ","creatorNameLang":"ja"},{"creatorName":"マツオカ, タカアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Matsuoka, Taka-aki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヤマダ, テツヤ","creatorNameLang":"ja"},{"creatorName":"ヤマダ, テツヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamada, Tetsuya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ワタダ, ヒロタカ","creatorNameLang":"ja"},{"creatorName":"ワタダ, ヒロタカ","creatorNameLang":"ja-Kana"},{"creatorName":"Watada, Hirotaka","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"フジタニ, ヨシオ","creatorNameLang":"ja"},{"creatorName":"フジタニ, ヨシオ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujitani, Yoshio","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-07-13"}],"displaytype":"detail","filename":"diabetologia_64_12_2803.pdf","filesize":[{"value":"5.28 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2010057/files/diabetologia_64_12_2803.pdf"},"version_id":"06d756bd-92bd-4363-9f26-ab5efbf2b522"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Beta cell heterogeneity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Ca2+ imaging","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Diphtheria toxin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"GLUT2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Pancreatic polypeptide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Ppy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Single-cell RNA sequence","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Streptozotocin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"TSPAN8","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"UCN3","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterisation of Ppy-lineage cells clarifies the functional heterogeneity of pancreatic beta cells in mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterisation of Ppy-lineage cells clarifies the functional heterogeneity of pancreatic beta cells in mice","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-07-13"},"publish_date":"2022-07-13","publish_status":"0","recid":"2010057","relation_version_is_last":true,"title":["Characterisation of Ppy-lineage cells clarifies the functional heterogeneity of pancreatic beta cells in mice"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-29T07:18:46.341378+00:00"}