{"created":"2024-12-12T07:41:07.777130+00:00","id":2010085,"links":{},"metadata":{"_buckets":{"deposit":"df71642d-60f8-4a27-92fa-15748b829c15"},"_deposit":{"created_by":7,"id":"2010085","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2010085"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02010085","sets":["1713853213384:1713853295607"]},"author_link":["1421"],"control_number":"2010085","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-05-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageStart":"2853","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"Molecules","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ERp57, a member of the protein disulfide isomerase family, is a ubiquitous disulfide catalyst that functions in the oxidative folding of various clients in the mammalian endoplasmic reticulum (ER). In concert with ER lectin-like chaperones calnexin and calreticulin (CNX/CRT), ERp57 functions in virtually all folding stages from co-translation to post-translation, and thus plays a critical role in maintaining protein homeostasis, with direct implication for pathology. Here, we present mechanisms by which Ca2+ regulates the formation of the ERp57-calnexin complex. Biochemical and isothermal titration calorimetry analyses revealed that ERp57 strongly interacts with CNX via a non-covalent bond in the absence of Ca2+. The ERp57-CNX complex not only promoted the oxidative folding of human leukocyte antigen heavy chains, but also inhibited client aggregation. These results suggest that this complex performs both enzymatic and chaperoning functions under abnormal physiological conditions, such as Ca2+ depletion, to effectively guide proper oxidative protein folding. The findings shed light on the molecular mechanisms underpinning crosstalk between the chaperone network and Ca2+.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"14203049","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.3390/molecules26102853"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/molecules26102853","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"385895"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"タニカワ, ユウヤ","creatorNameLang":"ja"},{"creatorName":"タニカワ, ユウヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanikawa, Yuya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"カネムラ, シンゴ","creatorNameLang":"ja"},{"creatorName":"カネムラ, シンゴ","creatorNameLang":"ja-Kana"},{"creatorName":"Kanemura, Shingo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"イトウ, ダイ","creatorNameLang":"ja"},{"creatorName":"イトウ, ダイ","creatorNameLang":"ja-Kana"},{"creatorName":"Ito, Dai","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Lin, Yuxi","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"松﨑, 元紀","creatorNameLang":"ja"},{"creatorName":"マツサキ, モトノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Matsusaki, Motonori","creatorNameLang":"en"}],"familyNames":[{"familyName":"松﨑","familyNameLang":"ja"},{"familyName":"マツサキ","familyNameLang":"ja-Kana"},{"familyName":"Matsusaki","familyNameLang":"en"}],"givenNames":[{"givenName":"元紀","givenNameLang":"ja"},{"givenName":"モトノリ","givenNameLang":"ja-Kana"},{"givenName":"Motonori","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1421","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"373532/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/373532/profile-ja.html"},{"nameIdentifier":"90817040","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=90817040"}]},{"creatorNames":[{"creatorName":"クロキ, キミコ","creatorNameLang":"ja"},{"creatorName":"クロキ, キミコ","creatorNameLang":"ja-Kana"},{"creatorName":"Kuroki, Kimiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヤマグチ, ヒロシ","creatorNameLang":"ja"},{"creatorName":"ヤマグチ, ヒロシ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamaguchi, Hiroshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"マエナカ, カツミ","creatorNameLang":"ja"},{"creatorName":"マエナカ, カツミ","creatorNameLang":"ja-Kana"},{"creatorName":"Maenaka, Katsumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Lee, Young-Ho","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"イナバ, ケンジ","creatorNameLang":"ja"},{"creatorName":"イナバ, ケンジ","creatorNameLang":"ja-Kana"},{"creatorName":"Inaba, Kenji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"オクムラ, マサキ","creatorNameLang":"ja"},{"creatorName":"オクムラ, マサキ","creatorNameLang":"ja-Kana"},{"creatorName":"Okumura, Masaki","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-07-29"}],"displaytype":"detail","filename":"molecules_26_10_2853.pdf","filesize":[{"value":"2.63 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2010085/files/molecules_26_10_2853.pdf"},"version_id":"4da70813-a5ad-48f3-a175-d03de4de3565"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"endoplasmic reticulum","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"oxidative folding","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"chaperone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"calnexin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ERp57","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"human leukocyte antigen","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Ca2+","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Ca2+ Regulates ERp57-Calnexin Complex Formation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ca2+ Regulates ERp57-Calnexin Complex Formation","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-07-29"},"publish_date":"2022-07-29","publish_status":"0","recid":"2010085","relation_version_is_last":true,"title":["Ca2+ Regulates ERp57-Calnexin Complex Formation"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-02-03T00:14:27.994128+00:00"}