{"created":"2024-12-12T07:41:19.991298+00:00","id":2010088,"links":{},"metadata":{"_buckets":{"deposit":"65387a38-9ab3-41e3-9246-f3e1b39c6924"},"_deposit":{"created_by":7,"id":"2010088","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2010088"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02010088","sets":["1713853213384:1713853295607"]},"author_link":["1317","959"],"control_number":"2010088","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-03-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageStart":"1422","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"Nutrients","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cockscomb hydrolysate was found to have neurite outgrowth-promoting activity in PC12 cells. To investigate the neurite outgrowth-promoting compounds derived from cockscomb hydrolysate, bioassay-guided purification was carried out. Purified active fractions were obtained by liquid–liquid partition, followed by column chromatography. High-performance liquid chromatography and proton nuclear magnetic resonance analyses of the purified active fractions clarified that the main compounds are threonine, alanine, valine, and methionine. By screening for 20 kinds of amino acids, it was shown that valine and methionine, but not threonine and alanine, have neurite outgrowth-promoting activity. The results of activity evaluation of the mixture of amino acids indicated that alanine enhanced the activity of valine and that the mixture of valine and methionine showed a higher ratio of neurite formation than did each of them alone. On the other hand, dipeptides formed by valine and methionine showed weak neurite outgrowth-promoting activity. A mixture of threonine, alanine, valine, and methionine at the same concentrations as those in cockscomb hydrolysate showed neurite outgrowth-promoting activity comparable to that of cockscomb hydrolysate although threonine, alanine, valine, and methionine alone did not show activity at their concentrations in cockscomb hydrolysate. Therefore, the strong neurite outgrowth-promoting activity of cockscomb hydrolysate was considered to be due to the synergistic effect of threonine, alanine, valine, and methionine.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"20726643","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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Takeru","creatorNameLang":"en"}],"familyNames":[{"familyName":"古賀","familyNameLang":"ja"},{"familyName":"コガ","familyNameLang":"ja-Kana"},{"familyName":"Koga","familyNameLang":"en"}],"givenNames":[{"givenName":"武尊","givenNameLang":"ja"},{"givenName":"タケル","givenNameLang":"ja-Kana"},{"givenName":"Takeru","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1317","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"397831/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/397831/profile-ja.html"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"田井, 章博","creatorNameLang":"ja"},{"creatorName":"タイ, アキヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Tai, Akihiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"田井","familyNameLang":"ja"},{"familyName":"タイ","familyNameLang":"ja-Kana"},{"familyName":"Tai","familyNameLang":"en"}],"givenNames":[{"givenName":"章博","givenNameLang":"ja"},{"givenName":"アキヒロ","givenNameLang":"ja-Kana"},{"givenName":"Akihiro","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"959","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"350199/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/350199/profile-ja.html"},{"nameIdentifier":"70284081","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=70284081"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-07-29"}],"displaytype":"detail","filename":"nu_14_7_1422.pdf","filesize":[{"value":"1.82 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2010088/files/nu_14_7_1422.pdf"},"version_id":"5ec8f7fa-38d7-494a-89ff-308b3f3f1c98"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cockscomb hydrolysate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"amino acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PC12 cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"NGF","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"dibutyryl cyclic AMP","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Neurite Outgrowth-Promoting Compounds from Cockscomb Hydrolysate","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Neurite Outgrowth-Promoting Compounds from Cockscomb Hydrolysate","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-07-29"},"publish_date":"2022-07-29","publish_status":"0","recid":"2010088","relation_version_is_last":true,"title":["Neurite Outgrowth-Promoting Compounds from Cockscomb Hydrolysate"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-02-04T02:37:23.791598+00:00"}