{"created":"2024-12-12T07:50:01.272445+00:00","id":2010193,"links":{},"metadata":{"_buckets":{"deposit":"3b56262b-6710-4c66-b18b-78f24dd0b155"},"_deposit":{"created_by":7,"id":"2010193","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2010193"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02010193","sets":["1713853213384:1713853296295:1716267876683:1716268551628"]},"author_link":["785"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"αカテニンの適切な張力感受性は上皮スフェロイドの球形への形態形成を確実にする","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"Tension-sensitivity of α-catenin on morphogenesis","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-06-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"73","bibliographicPageStart":"55","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"Cell Structure and Function","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The adherens junction (AJ) is an actin filament-anchoring junction. It plays a central role in epithelial morphogenesis through cadherin-based recognition and adhesion among cells. The stability and plasticity of AJs are required for the morphogenesis. An actin-binding α-catenin is an essential component of the cadherin-catenin complex and functions as a tension transducer that changes its conformation and induces AJ development in response to tension. Despite much progress in understanding molecular mechanisms of tension sensitivity of α-catenin, its significance on epithelial morphogenesis is still unknown. Here we show that the tension sensitivity of α-catenin is essential for epithelial cells to form round spheroids through proper multicellular rearrangement. Using a novel in vitro suspension culture model, we found that epithelial cells form round spheroids even from rectangular-shaped cell masses with high aspect ratios without using high tension and that increased tension sensitivity of α-catenin affected this morphogenesis. Analyses of AJ formation and cellular tracking during rounding morphogenesis showed cellular rearrangement, probably through AJ remodeling. The rearrangement occurs at the cell mass level, but not single-cell level. Hypersensitive α-catenin mutant-expressing cells did not show cellular rearrangement at the cell mass level, suggesting that the appropriate tension sensitivity of α-catenin is crucial for the coordinated round morphogenesis.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan Society for Cell Biology","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an open access article distributed under the terms of the Creative Commons BY (Attribution) License (https://creativecommons.org/licenses/by/4.0/legalcode), which permits the unrestricted distribution, reproduction and use of the article provided the original source and authors are credited.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"13473700","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"03867196","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA0060007X","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3644号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-07-28"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開\n本論文は，著者Ryosuke Nishimuraの学位論文として提出され，学位審査・授与の対象となっている。\n学位授与者所属 : 徳島大学大学院医学研究科（医学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲医第1537号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1247/csf.22014"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1247/csf.22014","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"388780"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"西村, 亮祐","creatorNameLang":"ja"},{"creatorName":"ニシムラ, リョウスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Nishimura, Ryosuke","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"カトウ, カガヤキ","creatorNameLang":"ja"},{"creatorName":"カトウ, カガヤキ","creatorNameLang":"ja-Kana"},{"creatorName":"Kato, Kagayaki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"サイダ, ミサコ","creatorNameLang":"ja"},{"creatorName":"サイダ, ミサコ","creatorNameLang":"ja-Kana"},{"creatorName":"Saida, Misako","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"カメイ, ヤスヒロ","creatorNameLang":"ja"},{"creatorName":"カメイ, ヤスヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Kamei, Yasuhiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"タケダ, マサヒロ","creatorNameLang":"ja"},{"creatorName":"タケダ, マサヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Takeda, Masahiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ミヨシ, ヒロミ","creatorNameLang":"ja"},{"creatorName":"ミヨシ, ヒロミ","creatorNameLang":"ja-Kana"},{"creatorName":"Miyoshi, Hiromi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヤマガタ, ユタカ","creatorNameLang":"ja"},{"creatorName":"ヤマガタ, ユタカ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamagata, Yutaka","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"アマノ, ユウ","creatorNameLang":"ja"},{"creatorName":"アマノ, ユウ","creatorNameLang":"ja-Kana"},{"creatorName":"Amano, Yu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"米村, 重信","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"785","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-08-25"}],"displaytype":"detail","filename":"k3644_abstract.pdf","filesize":[{"value":"92.7 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MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2010193/files/k3644_fulltext.pdf"},"version_id":"7df101c6-8938-4915-bbd4-83134a53f1af"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"αカテニン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ビンキュリン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞間接着","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"上皮形態形成","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"メカノトランスダクション","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"α-catenin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"vinculin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"adherens junction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"morphogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mechanotransduction","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Appropriate tension sensitivity of α-catenin ensures rounding morphogenesis of epithelial spheroids","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Appropriate tension sensitivity of α-catenin ensures rounding morphogenesis of epithelial spheroids","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268551628"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-08-25"},"publish_date":"2022-08-25","publish_status":"0","recid":"2010193","relation_version_is_last":true,"title":["Appropriate tension sensitivity of α-catenin ensures rounding morphogenesis of epithelial spheroids"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-12-12T07:50:10.805428+00:00"}