{"created":"2024-12-12T07:55:34.292941+00:00","id":2010258,"links":{},"metadata":{"_buckets":{"deposit":"f37cf29f-b8cd-4d65-af72-3420753bcc15"},"_deposit":{"created_by":7,"id":"2010258","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2010258"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02010258","sets":["1713853213384:1713853295607"]},"author_link":["857","839"],"control_number":"2010258","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"EP2, but not EP4, steadily stimulates the Gαs-protein pathway","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-02-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"783","bibliographicPageStart":"775","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"FEBS Open Bio","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"EP2 and EP4 prostanoid receptors have long been considered to have similar roles, since they are known to couple with Gαs-protein and activate cAMP-mediated signaling pathways. In this study, we re-evaluated the results of cAMP assays with or without phosphodiesterase (PDE) inhibitor pretreatment. Here, we show that in the absence of PDE inhibitor pretreatment, prostaglandin E2 causes accumulation of cAMP in EP2 receptors, whereas markedly low levels of cAMP accumulated in EP4 receptors. By applying the Black/Leff operational model calculation, we found that EP2 receptors have a biased ability to intrinsically activate the Gαs-protein-mediated pathway, whereas EP4 receptors have strong biased activity for the Gαi-protein-mediated pathway. Thus, EP2 and EP4 receptors may not be similar Gαs-coupled receptors but instead substantially different receptors with distinct roles.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"John Wiley & Sons","subitem_publisher_language":"en"},{"subitem_publisher":"Federation of European Biochemical Societies","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"22115463","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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Keijo","creatorNameLang":"en"}],"familyNames":[{"familyName":"福島","familyNameLang":"ja"},{"familyName":"フクシマ","familyNameLang":"ja-Kana"},{"familyName":"Fukushima","familyNameLang":"en"}],"givenNames":[{"givenName":"圭穣","givenNameLang":"ja"},{"givenName":"ケイジョウ","givenNameLang":"ja-Kana"},{"givenName":"Keijo","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"857","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"323728/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/323728/profile-ja.html"},{"nameIdentifier":"10805112","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=10805112"}]},{"creatorNames":[{"creatorName":"セノオ, カナホ","creatorNameLang":"ja"},{"creatorName":"セノオ, カナホ","creatorNameLang":"ja-Kana"},{"creatorName":"Senoo, Kanaho","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"クラタ, ナオキ","creatorNameLang":"ja"},{"creatorName":"クラタ, ナオキ","creatorNameLang":"ja-Kana"},{"creatorName":"Kurata, Naoki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Regan, John W.","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"藤野, 裕道","creatorNameLang":"ja"},{"creatorName":"フジノ, ヒロミチ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujino, Hiromichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"藤野","familyNameLang":"ja"},{"familyName":"フジノ","familyNameLang":"ja-Kana"},{"familyName":"Fujino","familyNameLang":"en"}],"givenNames":[{"givenName":"裕道","givenNameLang":"ja"},{"givenName":"ヒロミチ","givenNameLang":"ja-Kana"},{"givenName":"Hiromichi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"839","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"313992/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/313992/profile-ja.html"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-09-20"}],"displaytype":"detail","filename":"fob_12_4_775.pdf","filesize":[{"value":"586 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receptor","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The Gαs-protein-mediated pathway may be steadily stimulated by prostanoid EP2 receptors, but not by EP4 receptors","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Gαs-protein-mediated pathway may be steadily stimulated by prostanoid EP2 receptors, but not by EP4 receptors","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-09-20"},"publish_date":"2022-09-20","publish_status":"0","recid":"2010258","relation_version_is_last":true,"title":["The Gαs-protein-mediated pathway may be steadily stimulated by prostanoid EP2 receptors, but not by EP4 receptors"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-29T12:32:58.145039+00:00"}