{"created":"2024-12-12T07:59:05.197409+00:00","id":2010307,"links":{},"metadata":{"_buckets":{"deposit":"9778b657-a1f7-4d90-ac42-aaea2ed9074c"},"_deposit":{"created_by":7,"id":"2010307","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2010307"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02010307","sets":["1713853213384:1713853295607"]},"author_link":["300"],"control_number":"2010307","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-08-19","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"188","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"BMC Oral Health","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: We previously showed that nasal administration of a combination of dendritic cell (DC) targeted DNA plasmid expressing Flt3 ligand and CpG oligodeoxynucleotides 1826 as a mucosal adjuvant (double adjuvant, DA) provoked protective immunity in the upper respiratory tract of young adult and aging mice. Here, we investigated whether the nasal DA system induces secretory (S)IgA antibodies (Abs) toward recombinant fimbrillin (rFimA) of Porphyromonas gingivalis (P. gingivalis) in the saliva of young adult and aging mice. Further, we examined the functional applicability of rFimA-specific salivary SIgA Abs.\nMethods: BALB/c mice (8- or 48-week-old) were nasally immunized with rFimA plus DA three times at weekly intervals. Control mice were nasally administered rFimA alone. Saliva samples were collected 1 week after the final immunization, and were subjected to rFimA-specific ELISA. To examine the functional applicability of rFimA-specific SIgA Abs, IgA-enriched saliva samples were subjected to an inhibition assay in order to assess the numbers of P. gingivalis cells bound to the salivary protein statherin.\nResults: The 8- and 48-week-old mice administered nasal rFimA plus DA showed significantly increased levels of rFimA-specific SIgA Abs in saliva and elevated numbers of CD11c+ DCs in sublingual glands (SLGs), periglandular lymph nodes (PGLNs) and submandibular glands (SMGs) as well as nasopharyngeal-associated lymphoid tissues (NALT) compared to mice administered rFimA alone. Further, rFimA-specific SIgA Abs-containing saliva, in which IgG Abs of 8- and 48-week-old mice administered nasal rFimA plus DA were removed, significantly inhibited binding of P. gingivalis to the salivary protein.\nConclusions: These findings show that this DA system could be an effective nasal vaccine strategy for the enhancement of P. gingivalis-specific protective immunity in the oral cavity of adolescents and older individuals.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature","subitem_publisher_language":"en"},{"subitem_publisher":"BioMed Central","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"14726831","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11839983","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1186/s12903-019-0886-2"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1186/s12903-019-0886-2","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"374191"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"コブチ, ケンジロウ","creatorNameLang":"ja"},{"creatorName":"コブチ, ケンジロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Kobuchi, Kenjiro","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"片岡, 宏介","creatorNameLang":"ja"},{"creatorName":"カタオカ, コウスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Kataoka, Kosuke","creatorNameLang":"en"}],"familyNames":[{"familyName":"片岡","familyNameLang":"ja"},{"familyName":"カタオカ","familyNameLang":"ja-Kana"},{"familyName":"Kataoka","familyNameLang":"en"}],"givenNames":[{"givenName":"宏介","givenNameLang":"ja"},{"givenName":"コウスケ","givenNameLang":"ja-Kana"},{"givenName":"Kosuke","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"300","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"172135/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/172135/profile-ja.html"},{"nameIdentifier":"50283792","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=50283792"}]},{"creatorNames":[{"creatorName":"タグチ, ヨウイチロウ","creatorNameLang":"ja"},{"creatorName":"タグチ, ヨウイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Taguchi, Yoichiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ミヤケ, タツロウ","creatorNameLang":"ja"},{"creatorName":"ミヤケ, タツロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Miyake, Tatsuro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ウメダ, マコト","creatorNameLang":"ja"},{"creatorName":"ウメダ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"Umeda, Makoto","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-09-27"}],"displaytype":"detail","filename":"BMCOH_19_188.pdf","filesize":[{"value":"1.06 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2010307/files/BMCOH_19_188.pdf"},"version_id":"514fc0a0-bef1-4a33-b140-dd26754b0ca7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CpG ODN","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Dendritic cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA plasmid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"FimA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"FL gene","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Nasal adjuvant","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Nasal immunization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Salivary SIgA","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Nasal double DNA adjuvant induces salivary FimA-specific secretory IgA antibodies in young and aging mice and blocks Porphyromonas gingivalis binding to a salivary protein","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Nasal double DNA adjuvant induces salivary FimA-specific secretory IgA antibodies in young and aging mice and blocks Porphyromonas gingivalis binding to a salivary protein","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-09-27"},"publish_date":"2022-09-27","publish_status":"0","recid":"2010307","relation_version_is_last":true,"title":["Nasal double DNA adjuvant induces salivary FimA-specific secretory IgA antibodies in young and aging mice and blocks Porphyromonas gingivalis binding to a salivary protein"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-27T06:44:00.892092+00:00"}