{"created":"2024-12-12T08:02:31.225492+00:00","id":2010353,"links":{},"metadata":{"_buckets":{"deposit":"7fedc434-ac91-40ae-b4ea-dd6d40729d42"},"_deposit":{"created_by":7,"id":"2010353","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2010353"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02010353","sets":["1713853213384:1713853295607"]},"author_link":["306"],"control_number":"2010353","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-12-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"23","bibliographicPageStart":"13159","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"International Journal of Molecular Sciences","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Genomic DNA methylation is involved in many diseases and is expected to be a specific biomarker for even the pre-symptomatic diagnosis of many diseases. Thus, a rapid and inexpensive detection method is required for disease diagnosis. We have previously reported that cytosine methylation in G-quadruplex (G4)-forming oligonucleotides develops different G4 topologies. In this study, we developed a method for detecting CpG methylation in G4-forming oligonucleotides based on the structural differences between methylated and unmethylated G4 DNAs. The differences in G4 topologies due to CpG methylation can be discriminated by G4 ligands. We performed a binding assay between methylated or unmethylated G4 DNAs and G4 ligands. The binding abilities of fluorescent G4 ligands to BCL-2, HRAS1, HRAS2, VEGF G4-forming sequences were examined by fluorescence-based microtiter plate assay. The differences in fluorescence intensities between methylated and unmethylated G4 DNAs were statistically significant. In addition to fluorescence detection, the binding of G4 ligand to DNA was detected by chemiluminescence. A significant difference was also detected in chemiluminescence intensity between methylated and unmethylated DNA. This is the first study on the detection of CpG methylation in G4 structures, focusing on structural changes using G4 ligands.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"14220067","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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カオリ","creatorNameLang":"ja"},{"creatorName":"ツカコシ, カオリ","creatorNameLang":"ja-Kana"},{"creatorName":"Tsukakoshi, Kaori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ma, Yue","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ナガサワ, カズオ","creatorNameLang":"ja"},{"creatorName":"ナガサワ, カズオ","creatorNameLang":"ja-Kana"},{"creatorName":"Nagasawa, Kazuo","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"沼田, 周助","creatorNameLang":"ja"},{"creatorName":"ヌマタ, シュウスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Numata, Shusuke","creatorNameLang":"en"}],"familyNames":[{"familyName":"沼田","familyNameLang":"ja"},{"familyName":"ヌマタ","familyNameLang":"ja-Kana"},{"familyName":"Numata","familyNameLang":"en"}],"givenNames":[{"givenName":"周助","givenNameLang":"ja"},{"givenName":"シュウスケ","givenNameLang":"ja-Kana"},{"givenName":"Shusuke","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"306","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"172447/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/172447/profile-ja.html"},{"nameIdentifier":"10403726","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=10403726"}]},{"creatorNames":[{"creatorName":"イノウエ, ユウキ","creatorNameLang":"ja"},{"creatorName":"イノウエ, ユウキ","creatorNameLang":"ja-Kana"},{"creatorName":"Inoue, Yuuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kim, Yeji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"イケブクロ, カズノリ","creatorNameLang":"ja"},{"creatorName":"イケブクロ, カズノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Ikebukuro, Kazunori","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-10-28"}],"displaytype":"detail","filename":"ijms_22_23_13159.pdf","filesize":[{"value":"1.63 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2010353/files/ijms_22_23_13159.pdf"},"version_id":"aef0a55c-544b-4415-b787-8af2e88ba24e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"DNA methylation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"G-quadruplex","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"G4 ligand","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"biosensor","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Detection of CpG Methylation in G-Quadruplex Forming Sequences Using G-Quadruplex Ligands","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Detection of CpG Methylation in G-Quadruplex Forming Sequences Using G-Quadruplex Ligands","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-10-28"},"publish_date":"2022-10-28","publish_status":"0","recid":"2010353","relation_version_is_last":true,"title":["Detection of CpG Methylation in G-Quadruplex Forming Sequences Using G-Quadruplex Ligands"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-27T06:47:59.551213+00:00"}