{"created":"2024-12-12T08:15:22.859418+00:00","id":2010520,"links":{},"metadata":{"_buckets":{"deposit":"aa064de5-bc0c-40dc-b4e9-66f65ea290b1"},"_deposit":{"created_by":7,"id":"2010520","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2010520"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02010520","sets":["1713853213384:1713853295607"]},"author_link":["772","747","891"],"control_number":"2010520","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-02-28","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"100241","bibliographicVolumeNumber":"16","bibliographic_titles":[{"bibliographic_title":"Veterinary and Animal Science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Mosaicism, including alleles comprising both wild-type and mutant, is a serious problem for gene modification by gene editing using electroporation. One-step generation of F0 pigs with completely desired gene modifications saves cost and time, but the major obstacles have been mosaic mutations. We hypothesized that the timing of electroporation prior to in vitro fertilization (IVF) can increase the rates of biallelic mutation for multiple gene knockout as the permeability of mature oocytes is greater than that of zygotes. Hence, we determined whether the timing of electroporation during in vitro maturation (IVM) culture enhances triple gene editing in the resulting blastocysts. Three gRNAs targeting KDR, PDX1, and SALL1 were simultaneously introduced into the oocytes that had been incubated for 40, 42, and 44 h from the start of the IVM culture. Electroporation with three gRNAs at 40 h and 42 h during IVM culture decreased the blastocyst formation rates and did not improve the mutation rates and target number of biallelic mutations in the resulting blastocysts. The blastocyst formation rate, mutation rates, and target numbers in the resulting blastocysts from oocytes treated by electroporation at 44 h of IVM culture were similar to those of control zygotes electroporated at 13 h after the initiation of IVF. In conclusion, multiple gene editing efficiency in the resulting blastocysts was comparable between oocytes electroporated before and after the fertilization, indicating that oocytes with completed maturation time may allow better functioning of materials accepting gene editing application.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"2451943X","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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コウキ","creatorNameLang":"ja"},{"creatorName":"タケバヤシ, コウキ","creatorNameLang":"ja-Kana"},{"creatorName":"Takebayashi, Koki","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"平田, 真樹","creatorNameLang":"ja"},{"creatorName":"ヒラタ, マキ","creatorNameLang":"ja-Kana"},{"creatorName":"Hirata, Maki","creatorNameLang":"en"}],"familyNames":[{"familyName":"平田","familyNameLang":"ja"},{"familyName":"ヒラタ","familyNameLang":"ja-Kana"},{"familyName":"Hirata","familyNameLang":"en"}],"givenNames":[{"givenName":"真樹","givenNameLang":"ja"},{"givenName":"マキ","givenNameLang":"ja-Kana"},{"givenName":"Maki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"891","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"327914/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/327914/profile-ja.html"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"谷原, 史倫","creatorNameLang":"ja"},{"creatorName":"タニハラ, フミノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanihara, Fuminori","creatorNameLang":"en"}],"familyNames":[{"familyName":"谷原","familyNameLang":"ja"},{"familyName":"タニハラ","familyNameLang":"ja-Kana"},{"familyName":"Tanihara","familyNameLang":"en"}],"givenNames":[{"givenName":"史倫","givenNameLang":"ja"},{"givenName":"フミノリ","givenNameLang":"ja-Kana"},{"givenName":"Fuminori","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"747","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90754680","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=90754680"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"音井, 威重","creatorNameLang":"ja"},{"creatorName":"オトイ, タケシゲ","creatorNameLang":"ja-Kana"},{"creatorName":"Otoi, Takeshige","creatorNameLang":"en"}],"familyNames":[{"familyName":"音井","familyNameLang":"ja"},{"familyName":"オトイ","familyNameLang":"ja-Kana"},{"familyName":"Otoi","familyNameLang":"en"}],"givenNames":[{"givenName":"威重","givenNameLang":"ja"},{"givenName":"タケシゲ","givenNameLang":"ja-Kana"},{"givenName":"Takeshige","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"772","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"292974/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/292974/profile-ja.html"},{"nameIdentifier":"30311814","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=30311814"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-01-18"}],"displaytype":"detail","filename":"vas_16_100241.pdf","filesize":[{"value":"578 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knockout","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Permeability","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Pig","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-01-18"},"publish_date":"2023-01-18","publish_status":"0","recid":"2010520","relation_version_is_last":true,"title":["Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-29T13:08:27.632958+00:00"}