{"created":"2024-12-12T08:15:33.300315+00:00","id":2010522,"links":{},"metadata":{"_buckets":{"deposit":"8fe95e90-d87f-494d-8cfd-de9e7d068699"},"_deposit":{"created_by":7,"id":"2010522","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2010522"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02010522","sets":["1713853213384:1713853295607"]},"author_link":["1449"],"control_number":"2010522","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-10-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageStart":"2024","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"Viruses","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Laryngeal papilloma (LP), which is associated with infection by human papillomavirus (HPV)-6 or -11, displays aggressive growth. The precise molecular mechanism underlying the tumorigenesis of LP has yet to be uncovered. Building on our earlier research into HPV-6, in this study, the viral gene expression of HPV-11 was investigated by quantitative PCR and DNA/RNA in situ hybridization. Additionally, newly developed antibodies against the E4 protein of HPV-6 and HPV-11 were evaluated by immunohistochemistry. The average viral load of HPV-11 in LP was 1.95 ± 0.66 × 105 copies/ng DNA, and 88% of HPV mRNA expression was found to be E4, E5a, and E5b mRNAs. According to RNA in situ hybridization, E4 and E5b mRNAs were expressed from the middle to upper part of the epithelium. E4 immunohistochemistry revealed a wide positive reaction in the upper cell layer in line with E4 mRNA expression. Other head and neck lesions with HPV-11 infection also showed a positive reaction in E4 immunohistochemistry. The distribution pattern of HPV DNA, viral mRNA, and E4 protein in LP with HPV-11 infection was quite similar to that of HPV-6. Therefore, it might be possible to apply these E4-specific antibodies in other functional studies as well as clinical applications, including targeted molecular therapies in patients with HPV-6 and HPV-11 infection.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"19994915","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.3390/v13102024"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/v13102024","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"388411"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"イケガミ, タロウ","creatorNameLang":"ja"},{"creatorName":"イケガミ, タロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Ikegami, Taro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"キセ, ノリモト","creatorNameLang":"ja"},{"creatorName":"キセ, ノリモト","creatorNameLang":"ja-Kana"},{"creatorName":"Kise, Norimoto","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"キンジョウ, ヒデトシ","creatorNameLang":"ja"},{"creatorName":"キンジョウ, ヒデトシ","creatorNameLang":"ja-Kana"},{"creatorName":"Kinjyo, Hidetoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"コンドウ, シュンスケ","creatorNameLang":"ja"},{"creatorName":"コンドウ, シュンスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Kondo, Shunsuke","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"スズキ, ミキオ","creatorNameLang":"ja"},{"creatorName":"スズキ, ミキオ","creatorNameLang":"ja-Kana"},{"creatorName":"Suzuki, Mikio","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ツカハラ, ナルトシ","creatorNameLang":"ja"},{"creatorName":"ツカハラ, ナルトシ","creatorNameLang":"ja-Kana"},{"creatorName":"Tsukahara, Narutoshi","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"村上, 明一","creatorNameLang":"ja"},{"creatorName":"ムラカミ, アキカズ","creatorNameLang":"ja-Kana"},{"creatorName":"Murakami, Akikazu","creatorNameLang":"en"}],"familyNames":[{"familyName":"村上","familyNameLang":"ja"},{"familyName":"ムラカミ","familyNameLang":"ja-Kana"},{"familyName":"Murakami","familyNameLang":"en"}],"givenNames":[{"givenName":"明一","givenNameLang":"ja"},{"givenName":"アキカズ","givenNameLang":"ja-Kana"},{"givenName":"Akikazu","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1449","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"376683/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/376683/profile-ja.html"},{"nameIdentifier":"00733635","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=00733635"}]},{"creatorNames":[{"creatorName":"キユナ, アサノリ","creatorNameLang":"ja"},{"creatorName":"キユナ, アサノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Kiyuna, Asanori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"アゲナ, シンヤ","creatorNameLang":"ja"},{"creatorName":"アゲナ, シンヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Agena, Shinya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"タナカ, カツノリ","creatorNameLang":"ja"},{"creatorName":"タナカ, カツノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanaka, Katsunori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ハセガワ, ナルミ","creatorNameLang":"ja"},{"creatorName":"ハセガワ, ナルミ","creatorNameLang":"ja-Kana"},{"creatorName":"Hasegawa, Narumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"カワカミ, ジュンコ","creatorNameLang":"ja"},{"creatorName":"カワカミ, ジュンコ","creatorNameLang":"ja-Kana"},{"creatorName":"Kawakami, Junko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ガナハ, アキラ","creatorNameLang":"ja"},{"creatorName":"ガナハ, アキラ","creatorNameLang":"ja-Kana"},{"creatorName":"Ganaha, Akira","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"マエダ, ヒロユキ","creatorNameLang":"ja"},{"creatorName":"マエダ, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Maeda, Hiroyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヒラカワ, ヒトシ","creatorNameLang":"ja"},{"creatorName":"ヒラカワ, ヒトシ","creatorNameLang":"ja-Kana"},{"creatorName":"Hirakawa, Hitoshi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-01-25"}],"displaytype":"detail","filename":"virus_13_10_2024.pdf","filesize":[{"value":"2.77 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2010522/files/virus_13_10_2024.pdf"},"version_id":"be57ae87-a6b6-4b66-b979-a4abcbd0e278"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"laryngeal papilloma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"human papillomavirus 6","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"human papillomavirus 11","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"viral DNA and mRNA expression","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"anti-E4 antibody","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development of Antibodies against HPV-6 and HPV-11 for the Study of Laryngeal Papilloma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of Antibodies against HPV-6 and HPV-11 for the Study of Laryngeal Papilloma","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-01-25"},"publish_date":"2023-01-25","publish_status":"0","recid":"2010522","relation_version_is_last":true,"title":["Development of Antibodies against HPV-6 and HPV-11 for the Study of Laryngeal Papilloma"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-02-12T00:39:28.367404+00:00"}