{"created":"2024-12-12T08:18:17.822588+00:00","id":2010556,"links":{},"metadata":{"_buckets":{"deposit":"2679230f-2e34-4b58-b2d3-8d9d17829a27"},"_deposit":{"created_by":7,"id":"2010556","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2010556"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02010556","sets":["1713853213384:1713853295607"]},"author_link":["1472"],"control_number":"2010556","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Oxidative Deamination Activity of EGCG","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-04-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageStart":"e0153002","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"PLOS ONE","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"(-)-Epigallocatechin-3-O-gallate (EGCG), the most abundant polyphenol in green tea, mediates the oxidative modification of proteins, generating protein carbonyls. However, the underlying molecular mechanism remains unclear. Here we analyzed the EGCG-derived intermediates generated upon incubation with the human serum albumin (HSA) and established that EGCG selectively oxidized the lysine residues via its oxidative deamination activity. In addition, we characterized the EGCG-oxidized proteins and discovered that the EGCG could be an endogenous source of the electrically-transformed proteins that could be recognized by the natural antibodies. When HSA was incubated with EGCG in the phosphate-buffered saline (pH 7.4) at 37°C, the protein carbonylation was associated with the formation of EGCG-derived products, such as the protein-bound EGCG, oxidized EGCG, and aminated EGCG. The aminated EGCG was also detected in the sera from the mice treated with EGCG in vivo. EGCG selectively oxidized lysine residues at the EGCG-binding domains in HSA to generate an oxidatively deaminated product, aminoadipic semialdehyde. In addition, EGCG treatment results in the increased negative charge of the protein due to the oxidative deamination of the lysine residues. More strikingly, the formation of protein carbonyls by EGCG markedly increased its cross-reactivity with the natural IgM antibodies. These findings suggest that many of the beneficial effects of EGCG may be partly attributed to its oxidative deamination activity, generating the oxidized proteins as a target of natural antibodies.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"PLOS","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"19326203","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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マイ","creatorNameLang":"ja"},{"creatorName":"フルハシ, マイ","creatorNameLang":"ja-Kana"},{"creatorName":"Furuhashi, Mai","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ナカシマ, フミエ","creatorNameLang":"ja"},{"creatorName":"ナカシマ, フミエ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakashima, Fumie","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"シバタ, タカヒロ","creatorNameLang":"ja"},{"creatorName":"シバタ, タカヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Shibata, Takahiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"コンドウ, タツヒコ","creatorNameLang":"ja"},{"creatorName":"コンドウ, タツヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Kondo, Tatsuhiko","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"赤川, 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