{"created":"2024-12-12T09:04:47.054791+00:00","id":2010643,"links":{},"metadata":{"_buckets":{"deposit":"a0e02603-f66c-4aad-b193-4060ca92e34e"},"_deposit":{"created_by":7,"id":"2010643","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2010643"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02010643","sets":["1713853213384:1713853295607"]},"author_link":["1556","1521"],"control_number":"2010643","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-09-24","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"240","bibliographicPageStart":"226","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"Molecular Therapy - Nucleic Acids","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"mRNA and lipid nanoparticles have emerged as powerful systems for the preparation of vaccines against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. The emergence of novel variants or the necessity of cold chain logistics for approved mRNA vaccines undermines the investigation of next-generation systems that could preserve both potency and stability. However, the correlation between lipid nanoparticle composition and activity is not fully explored. Here, we screened a panel of ionizable lipids in vivo and identified lead lipid nanoparticles with a branched-tail lipid structure. Buffer optimization allowed the determination of lyophilization conditions, where lipid nanoparticle-encapsulated mRNA encoding SARS-CoV-2 spike protein could induce robust immunogenicity in mice after 1 month of storage at 5°C and 25°C. Intramuscularly injected lipid nanoparticles distributed in conventional dendritic cells in mouse lymph nodes induced balanced T helper (Th) 1/Th2 responses against SARS-CoV-2 spike protein. In nonhuman primates, two doses of 10 or 100 μg of mRNA induced higher spike-specific binding geometric mean titers than those from a panel of SARS-CoV-2-convalescent human sera. Immunized sera broadly inhibited the viral entry receptor angiotensin-converting enzyme 2 (ACE2) from binding to the spike protein in all six strains tested, including variants of concern. These results could provide useful information for designing next-generation mRNA vaccines.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The American Society of Gene and Cell Therapy","subitem_publisher_language":"en"},{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"21622531","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1016/j.omtn.2022.09.017"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.omtn.2022.09.017","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"392461"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"スズキ, ユウタ","creatorNameLang":"ja"},{"creatorName":"スズキ, ユウタ","creatorNameLang":"ja-Kana"},{"creatorName":"Suzuki, Yuta","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ミヤザキ, タカユキ","creatorNameLang":"ja"},{"creatorName":"ミヤザキ, タカユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Miyazaki, Takayuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ムトウ, ヒロキ","creatorNameLang":"ja"},{"creatorName":"ムトウ, ヒロキ","creatorNameLang":"ja-Kana"},{"creatorName":"Muto, Hiroki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"クバラ, ケンジ","creatorNameLang":"ja"},{"creatorName":"クバラ, ケンジ","creatorNameLang":"ja-Kana"},{"creatorName":"Kubara, Kenji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ムカイ, ヨウヘイ","creatorNameLang":"ja"},{"creatorName":"ムカイ, ヨウヘイ","creatorNameLang":"ja-Kana"},{"creatorName":"Mukai, Yohei","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ワタリ, リュウジ","creatorNameLang":"ja"},{"creatorName":"ワタリ, リュウジ","creatorNameLang":"ja-Kana"},{"creatorName":"Watari, Ryuji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"サトウ, シンヤ","creatorNameLang":"ja"},{"creatorName":"サトウ, シンヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Sato, Shinya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"コンドウ, ケイタ","creatorNameLang":"ja"},{"creatorName":"コンドウ, ケイタ","creatorNameLang":"ja-Kana"},{"creatorName":"Kondo, Keita","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"九十九, 伸一","creatorNameLang":"ja"},{"creatorName":"ツクモ, シンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"Tsukumo, Shin-ichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"九十九","familyNameLang":"ja"},{"familyName":"ツクモ","familyNameLang":"ja-Kana"},{"familyName":"Tsukumo","familyNameLang":"en"}],"givenNames":[{"givenName":"伸一","givenNameLang":"ja"},{"givenName":"シンイチ","givenNameLang":"ja-Kana"},{"givenName":"Shin-ichi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1556","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60092/profile-ja.html","nameIdentifierScheme":"徳島大学 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Koji","creatorNameLang":"en"}],"familyNames":[{"familyName":"安友","familyNameLang":"ja"},{"familyName":"ヤストモ","familyNameLang":"ja-Kana"},{"familyName":"Yasutomo","familyNameLang":"en"}],"givenNames":[{"givenName":"康二","givenNameLang":"ja"},{"givenName":"コウジ","givenNameLang":"ja-Kana"},{"givenName":"Koji","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1521","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60033/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/60033/profile-ja.html"},{"nameIdentifier":"30333511","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=30333511"}]},{"creatorNames":[{"creatorName":"イトウ, マサシ","creatorNameLang":"ja"},{"creatorName":"イトウ, マサシ","creatorNameLang":"ja-Kana"},{"creatorName":"Ito, Masashi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ツカハラ, カッペイ","creatorNameLang":"ja"},{"creatorName":"ツカハラ, カッペイ","creatorNameLang":"ja-Kana"},{"creatorName":"Tsukahara, Kappei","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-02-09"}],"displaytype":"detail","filename":"mtna_30_226.pdf","filesize":[{"value":"2.67 MB"}],"format":"application/pdf","licensetype":"license_5","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2010643/files/mtna_30_226.pdf"},"version_id":"1e1618d8-1b85-479b-a39d-d64b90e74b30"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Design and lyophilization of lipid nanoparticles for mRNA vaccine and its robust immune response in mice and nonhuman primates","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Design and lyophilization of lipid nanoparticles for mRNA vaccine and its robust immune response in mice and nonhuman primates","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-02-09"},"publish_date":"2023-02-09","publish_status":"0","recid":"2010643","relation_version_is_last":true,"title":["Design and lyophilization of lipid nanoparticles for mRNA vaccine and its robust immune response in mice and nonhuman primates"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-29T00:30:10.015841+00:00"}