{"created":"2024-12-12T09:35:51.297888+00:00","id":2010968,"links":{},"metadata":{"_buckets":{"deposit":"9d46e976-4492-4978-a13d-e07b80c8c245"},"_deposit":{"created_by":7,"id":"2010968","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2010968"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02010968","sets":["1713853213384:1713853296295:1716268427747:1716268703682"]},"author_link":["562","1629","1606"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"トロンビン活性化ヒト血小板における細胞内Ca2+動員と凝集に対するアスピリンおよびシロスタゾールの抑制効果","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"Ca2+ Mobilization and Aggregation in Platelets","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"100","bibliographicPageStart":"94","bibliographicVolumeNumber":"70","bibliographic_titles":[{"bibliographic_title":"The Journal of Medical Investigation","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Platelets play an important role in physiological hemostatic mechanisms. In contrast, platelet activation has been implicated in pathological conditions, such as atherosclerosis, angiogenesis, and inflammation. Thrombin is considered to be of particular pathological importance as a platelet-activating substance, and thrombin-activated platelets are detected in the blood of patients with advanced occlusive arterial disease. Ca2+ acts as a second messenger in platelet activation, and the regulation of intracellular Ca2+ concentrations ([Ca2+]i) is important for controlling platelet functions. However, changes in [Ca2+]i by antiplatelet agents remain unclear. Therefore, we herein investigated the relationship between [Ca2+]i and the intensity of platelet aggregation after a thrombin stimulation, the relationship between [Ca2+]i and the intensity of platelet aggregation by antiplatelet agents, and the effects of antiplatelet agents on thrombin-activated platelets as a surrogate platelet model for arterial occlusive disease. Fura2-loaded platelets were treated with phosphate-buffered saline or a low concentration of thrombin (0.005 U/mL), followed by antiplatelet agents (aspirin or cilostazol), and changes in [Ca2+]i and the intensity of platelet aggregation by the thrombin stimulation were measured using fluorescence spectrophotometry. Changes in [Ca2+]i and the intensity of platelet aggregation after the thrombin stimulation as well as the relationship between [Ca2+]i and the intensity of platelet aggregation by antiplatelet agents indicated that cilostazol exerted stronger antiplatelet effects than aspirin and also that antiplatelet effects may be attenuated in thrombin-activated platelets. The present results also suggest the utility of thrombin-activated platelets as a surrogate platelet model for arterial occlusive disease. These results may contribute to future drug development for antiplatelet therapy.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tokushima University Faculty of Medicine","subitem_publisher_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"13496867","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"13431420","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11166929","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3674号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（保健学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2023-02-16"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開\n本論文は，著者Atsumi Soneの学位論文として提出され，学位審査・授与の対象となっている。\n学位授与者所属 : 徳島大学大学院保健科学研究科（保健学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲保第57号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.2152/jmi.70.94"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2152/jmi.70.94","subitem_relation_type_select":"DOI"}}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"曽根, 淳美","creatorNameLang":"ja"},{"creatorName":"ソネ, アツミ","creatorNameLang":"ja-Kana"},{"creatorName":"Sone, Atsumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"安藝, 健作","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"562","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安井, 敏之","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1629","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"細井, 英司","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1606","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-05-15"}],"displaytype":"detail","filename":"k3674_abstract.pdf","filesize":[{"value":"110 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2010968/files/k3674_abstract.pdf"},"version_id":"7d95ed4a-4778-4be0-bab4-fcc37d8d02d5"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-05-15"}],"displaytype":"detail","filename":"k3674_review.pdf","filesize":[{"value":"150 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"other","url":"https://tokushima-u.repo.nii.ac.jp/record/2010968/files/k3674_review.pdf"},"version_id":"8165cfae-9427-40f0-9766-1017218d10e9"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-05-18"}],"displaytype":"detail","filename":"k3674_fulltext.pdf","filesize":[{"value":"601 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2010968/files/k3674_fulltext.pdf"},"version_id":"ba532960-bd80-432a-9fd0-358a3f5decc6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"intracellular Ca2+ concentration","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"intensity of platelet aggregation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"aspirin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cilostazol","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"thrombin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Inhibitory Effects of Aspirin and Cilostazol on Intracellular Ca2+ Mobilization and Aggregation in Thrombin-activated Human Platelets","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Inhibitory Effects of Aspirin and Cilostazol on Intracellular Ca2+ Mobilization and Aggregation in Thrombin-activated Human Platelets","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268703682"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-05-15"},"publish_date":"2023-05-15","publish_status":"0","recid":"2010968","relation_version_is_last":true,"title":["Inhibitory Effects of Aspirin and Cilostazol on Intracellular Ca2+ Mobilization and Aggregation in Thrombin-activated Human Platelets"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-12-12T09:36:06.008223+00:00"}