{"created":"2024-12-12T09:38:55.518684+00:00","id":2010995,"links":{},"metadata":{"_buckets":{"deposit":"4215d97a-bd4c-48a9-97e8-633130e55a38"},"_deposit":{"created_by":7,"id":"2010995","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2010995"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02010995","sets":["1713853213384:1713853295607"]},"author_link":["650"],"control_number":"2010995","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-11-23","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"7180","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"Nature Communications","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Calcareous soils cover one-third of all land and cause severe growth defects in plants due to the poor water solubility of iron at high pH. Poaceae species use a unique chelation strategy, whereby plants secrete a high-affinity metal chelator, known as phytosiderophores (mugineic acids), and reabsorb the iron-phytosiderophore complex by the yellow stripe 1/yellow stripe 1-like (YS1/YSL) transporter for efficient uptake of iron from the soil. Here, we present three cryo-electron microscopy structures of barley YS1 (HvYS1) in the apo state, in complex with an iron-phytosiderophore complex, Fe(III)-deoxymugineic acid (Fe(III)–DMA), and in complex with the iron-bound synthetic DMA analog (Fe(III)–PDMA). The structures reveal a homodimeric assembly mediated through an anti-parallel β-sheet interaction with cholesterol hemisuccinate. Each protomer adopts an outward open conformation, and Fe(III)–DMA is bound near the extracellular space in the central cavity. Fe(III)–PDMA occupies the same binding site as Fe(III)–DMA, demonstrating that PDMA can function as a potent fertilizer in an essentially identical manner to DMA. Our results provide a structural framework for iron-phytosiderophore recognition and transport by YS1/YSL transporters, which will enable the rational design of new, high-potency fertilizers.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"20411723","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA12645905","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1038/s41467-022-34930-1"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/s41467-022-34930-1","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"396500"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ヤマガタ, アツシ","creatorNameLang":"ja"},{"creatorName":"ヤマガタ, アツシ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamagata, Atsushi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ムラタ, ヨシコ","creatorNameLang":"ja"},{"creatorName":"ムラタ, ヨシコ","creatorNameLang":"ja-Kana"},{"creatorName":"Murata, Yoshiko","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"難波, 康祐","creatorNameLang":"ja"},{"creatorName":"ナンバ, コウスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Namba, Kosuke","creatorNameLang":"en"}],"familyNames":[{"familyName":"難波","familyNameLang":"ja"},{"familyName":"ナンバ","familyNameLang":"ja-Kana"},{"familyName":"Namba","familyNameLang":"en"}],"givenNames":[{"givenName":"康祐","givenNameLang":"ja"},{"givenName":"コウスケ","givenNameLang":"ja-Kana"},{"givenName":"Kosuke","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"650","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"262684/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/262684/profile-ja.html"},{"nameIdentifier":"50414123","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=50414123"}]},{"creatorNames":[{"creatorName":"テラダ, トオル","creatorNameLang":"ja"},{"creatorName":"テラダ, トオル","creatorNameLang":"ja-Kana"},{"creatorName":"Terada, Tohru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"フカイ, シュウヤ","creatorNameLang":"ja"},{"creatorName":"フカイ, シュウヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Fukai, Shuya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"シロウズ, ミカコ","creatorNameLang":"ja"},{"creatorName":"シロウズ, ミカコ","creatorNameLang":"ja-Kana"},{"creatorName":"Shirouzu, Mikako","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-06-02"}],"displaytype":"detail","filename":"ncomms_13_7180.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2010995/files/ncomms_13_7180.pdf"},"version_id":"585a4455-1df4-41a7-972e-514d78a44667"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Uptake mechanism of iron-phytosiderophore from the soil based on the structure of yellow stripe transporter","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Uptake mechanism of iron-phytosiderophore from the soil based on the structure of yellow stripe transporter","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-06-02"},"publish_date":"2023-06-02","publish_status":"0","recid":"2010995","relation_version_is_last":true,"title":["Uptake mechanism of iron-phytosiderophore from the soil based on the structure of yellow stripe transporter"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-28T01:46:33.544387+00:00"}