{"created":"2024-12-12T09:44:49.159985+00:00","id":2011047,"links":{},"metadata":{"_buckets":{"deposit":"0c649f5c-1ce2-45cc-815f-00a601b08bfa"},"_deposit":{"created_by":7,"id":"2011047","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2011047"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02011047","sets":["1713853213384:1713853296295:1716267878536:1716268699858"]},"author_link":["1513","516","567","1234","1579"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Vibrio vulnificus創傷感染モデルマウスにおける骨格筋内の特徴的な代謝変化","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"Metabolic Changing in V. vulnificus-Infected Tissue","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-03-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageStart":"e00682-22","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"mSystems","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Vibrio vulnificus is a bacterium that inhabits warm seawater or brackish water environments and causes foodborne diseases and wound infections. In severe cases, V. vulnificus invades the skeletal muscle tissue, where bacterial proliferation leads to septicemia and necrotizing fasciitis with high mortality. Despite this characteristic, information on metabolic changes in tissue infected with V. vulnificus is not available. Here, we elucidated the metabolic changes in V. vulnificus-infected mouse skeletal muscle using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). Metabolome analysis revealed changes in muscle catabolites and energy metabolites during V. vulnificus infection. In particular, succinic acid accumulated but fumaric acid decreased in the infected muscle. However, the virulence factor deletion mutant revealed that changes in metabolites and bacterial proliferation were abolished in skeletal muscle infected with a multifunctional-autoprocessing repeats-in-toxin (MARTX) mutant. On the other hand, mice that were immunosuppressed via cyclophosphamide (CPA) treatment exhibited a similar level of bacterial counts and metabolites between the wild type and MARTX mutant. Therefore, our data indicate that V. vulnificus induces metabolic changes in mouse skeletal muscle and proliferates by using the MARTX toxin to evade the host immune system. This study indicates a new correlation between V. vulnificus infections and metabolic changes that lead to severe reactions or damage to host skeletal muscle.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2023 Ishida et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. (https://creativecommons.org/licenses/by/4.0/)","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"23795077","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3734号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（栄養学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2023-03-24"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開\n本論文は，著者Kai Ishidaの学位論文として提出され，学位審査・授与の対象となっている。\n学位授与者所属 : 徳島大学大学院医科栄養学研究科（医科栄養学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲栄第299号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1128/msystems.00682-22"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1128/msystems.00682-22","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"397589"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石田, 快","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1513","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"下畑, 隆明","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"516","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"カンダ, ユウナ","creatorNameLang":"ja"},{"creatorName":"カンダ, ユウナ","creatorNameLang":"ja-Kana"},{"creatorName":"Kanda, Yuna","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nguyen, Anh Quoc","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"マスダ, ルミコ","creatorNameLang":"ja"},{"creatorName":"マスダ, ルミコ","creatorNameLang":"ja-Kana"},{"creatorName":"Masuda, Rumiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヤマザキ, コウヘイ","creatorNameLang":"ja"},{"creatorName":"ヤマザキ, コウヘイ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamazaki, Kohei","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"上番増, 喬","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"567","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"馬渡, 一諭","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1234","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"カシモト, タカシゲ","creatorNameLang":"ja"},{"creatorName":"カシモト, タカシゲ","creatorNameLang":"ja-Kana"},{"creatorName":"Kashimoto, Takashige","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"髙橋, 章","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1579","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-06-20"}],"displaytype":"detail","filename":"k3734_abstract_review.pdf","filesize":[{"value":"205 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2011047/files/k3734_abstract_review.pdf"},"version_id":"060dfd35-cdb3-4e7c-bac6-2bda501e11c3"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-06-20"}],"displaytype":"detail","filename":"k3734_fulltext.pdf","filesize":[{"value":"3.12 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2011047/files/k3734_fulltext.pdf"},"version_id":"45b47d53-76ca-49f3-9819-d4f54aa79c41"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Vibrio vulnificus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CE-TOFMS","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"host metabolomics","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"TCA cycle","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MARTX toxin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Characteristic Metabolic Changes in Skeletal Muscle due to Vibrio vulnificus Infection in a Wound Infection Model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characteristic Metabolic Changes in Skeletal Muscle due to Vibrio vulnificus Infection in a Wound Infection Model","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268699858"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-06-09"},"publish_date":"2023-06-09","publish_status":"0","recid":"2011047","relation_version_is_last":true,"title":["Characteristic Metabolic Changes in Skeletal Muscle due to Vibrio vulnificus Infection in a Wound Infection Model"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-12-12T09:45:00.833364+00:00"}