{"created":"2024-12-12T10:15:11.763437+00:00","id":2011370,"links":{},"metadata":{"_buckets":{"deposit":"56de90b5-3b78-41cc-8695-64077b485a81"},"_deposit":{"created_by":7,"id":"2011370","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2011370"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02011370","sets":["1713853213384:1713853295607"]},"author_link":["871","812"],"control_number":"2011370","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-10-11","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"744","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"BMC Oral Health","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background\nAnalyses of tooth families and tooth-forming units in medaka with regard to tooth replacement cycles and the localization of odontogenic stem cell niches in the pharyngeal dentition clearly indicate that continuous tooth replacement is maintained. The secretory calcium-binding phosphoprotein (scpp) gene cluster is involved in the formation of mineralized tissues, such as dental and bone tissues, and the genes encoding multiple SCPPs are conserved in fish, amphibians, reptiles, and mammals. In the present study, we examined the expression patterns of several scpp genes in the pharyngeal teeth of medaka to elucidate their roles during tooth formation and replacement.\nMethods\nHimedaka (Japanese medaka, Oryzias latipes) of both sexes (body length: 28 to 33 mm) were used in this study. Real-time quantitative reverse transcription-polymerase chain reaction (PCR) (qPCR) data were evaluated using one-way analysis of variance for multi-group comparisons, and the significance of differences was determined by Tukey’s comparison test. The expression of scpp genes was examined using in situ hybridization (ISH) with a digoxigenin-labeled, single-stranded antisense probe.\nResults\nqPCR results showed that several scpp genes were strongly expressed in pharyngeal tissues. ISH analysis revealed specific expression of scpp1, scpp5, and sparc in tooth germ, and scpp5 was continually expressed in the odontoblasts of teeth attached to pedicles, but not in the osteoblasts of pedicles. In addition, many scpp genes were expressed in inner dental epithelium (ide), but not in odontoblasts, and scpp2 consistently showed epithelial-specific expression in the functional teeth. Taken together, these data indicate that specific expression of scpp2 and scpp5 may play a critical role in pharyngeal tooth formation in medaka.\nConclusion\nWe characterized changes in the expression patterns of scpp genes in medaka during the formation and replacement of pharyngeal teeth.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central","subitem_publisher_language":"en"},{"subitem_publisher":"Springer Nature","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"14726831","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11839983","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1186/s12903-023-03498-7"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1186/s12903-023-03498-7","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"403421"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"守田, 剛","creatorNameLang":"ja"},{"creatorName":"モリタ, ツヨシ","creatorNameLang":"ja-Kana"},{"creatorName":"Morita, Tsuyoshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"守田","familyNameLang":"ja"},{"familyName":"モリタ","familyNameLang":"ja-Kana"},{"familyName":"Morita","familyNameLang":"en"}],"givenNames":[{"givenName":"剛","givenNameLang":"ja"},{"givenName":"ツヨシ","givenNameLang":"ja-Kana"},{"givenName":"Tsuyoshi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"871","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"323772/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/323772/profile-ja.html"},{"nameIdentifier":"40804513","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=40804513"}]},{"creatorNames":[{"creatorName":"マツモト, シン","creatorNameLang":"ja"},{"creatorName":"マツモト, シン","creatorNameLang":"ja-Kana"},{"creatorName":"Matsumoto, Shin","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"馬場, 麻人","creatorNameLang":"ja"},{"creatorName":"ババ, オト","creatorNameLang":"ja-Kana"},{"creatorName":"Baba, Otto","creatorNameLang":"en"}],"familyNames":[{"familyName":"馬場","familyNameLang":"ja"},{"familyName":"ババ","familyNameLang":"ja-Kana"},{"familyName":"Baba","familyNameLang":"en"}],"givenNames":[{"givenName":"麻人","givenNameLang":"ja"},{"givenName":"オト","givenNameLang":"ja-Kana"},{"givenName":"Otto","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"812","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"304903/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/304903/profile-ja.html"},{"nameIdentifier":"90251545","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=90251545"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-11-01"}],"displaytype":"detail","filename":"BMCOH_23_744.pdf","filesize":[{"value":"2.61 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2011370/files/BMCOH_23_744.pdf"},"version_id":"fa61eb45-4041-4c09-8907-c73382076d1b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Scpp","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Medaka","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Pharyngeal teeth","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"In situ hybridization","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Expression of secretory calcium-binding phosphoprotein (scpp) genes in medaka during the formation and replacement of pharyngeal teeth","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Expression of secretory calcium-binding phosphoprotein (scpp) genes in medaka during the formation and replacement of pharyngeal teeth","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-11-01"},"publish_date":"2023-11-01","publish_status":"0","recid":"2011370","relation_version_is_last":true,"title":["Expression of secretory calcium-binding phosphoprotein (scpp) genes in medaka during the formation and replacement of pharyngeal teeth"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-30T01:21:32.406989+00:00"}