{"created":"2025-01-14T00:51:00.581558+00:00","id":2011727,"links":{},"metadata":{"_buckets":{"deposit":"fdffcc4d-01ec-40f1-9e7e-0cc86975955d"},"_deposit":{"created_by":7,"id":"2011727","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2011727"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02011727","sets":["1713853213384:1713853295607"]},"author_link":["772"],"control_number":"2011727","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-06-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"31","bibliographicPageStart":"25","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"Regenerative Therapy","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Introduction: This study investigated the storage conditions under which cell aggregation occurs and the conditions that inhibit cell aggregation when human adipose tissue-derived mesenchymal stem cells (hADSCs) are stored in lactated Ringer’s solution (LR) supplemented with 3% trehalose and 5% dextran 40 (LR-3T-5D).\nMethods: We first examined the effects of storage temperature and time on the aggregation and viability of hADSCs stored in LR and LR-3T-5D. The cells were stored at 5 °C or 25 °C for various times up to 24 h. We then evaluated the effects of storage volume (250-2,000 μL), cell density (2.5-20 × 105 cells/mL), and nitrogen gas replacement on aggregation, oxygen partial pressure (pO2), and viability of hADSCs stored for 24 h at 25 °C in LR-3T-5D.\nResults: When stored in LR-3T-5D, viability did not change under either condition compared with pre-storage, but the cell aggregation rate increased significantly with storage at 25 °C for 24 h (p<0.001). In LR, the aggregation rate did not change under either condition, but cell viability decreased significantly after 24 h at both 5 °C and 25 °C (p < 0.05). The cell aggregation rates and pO2 tended to decrease with increasing solution volume and cell density. Nitrogen gas replacement significantly decreased the cell aggregation rate and pO2 (p < 0.05). However, there were no differences in viability among cells stored under conditions of different storage volumes, densities, and nitrogen gas replacement.\nConclusions: Aggregation of cells after storage at 25 °C in LR-3T-5D may be suppressed by increasing the storage volume and cell density as well as by incorporating nitrogen replacement, which lowers the pO2 in the solution.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Society of Regenerative Medicine","subitem_publisher_language":"en"},{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"23523204","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1016/j.reth.2023.05.002"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.reth.2023.05.002","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"398886"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"キクチ, タケシ","creatorNameLang":"ja"},{"creatorName":"キクチ, タケシ","creatorNameLang":"ja-Kana"},{"creatorName":"Kikuchi, Takeshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ニシムラ, マスヒロ","creatorNameLang":"ja"},{"creatorName":"ニシムラ, マスヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Nishimura, Masuhiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"シラカワ, チカゲ","creatorNameLang":"ja"},{"creatorName":"シラカワ, チカゲ","creatorNameLang":"ja-Kana"},{"creatorName":"Shirakawa, Chikage","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"フジタ, ヤスタカ","creatorNameLang":"ja"},{"creatorName":"フジタ, ヤスタカ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujita, Yasutaka","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"音井, 威重","creatorNameLang":"ja"},{"creatorName":"オトイ, タケシゲ","creatorNameLang":"ja-Kana"},{"creatorName":"Otoi, Takeshige","creatorNameLang":"en"}],"familyNames":[{"familyName":"音井","familyNameLang":"ja"},{"familyName":"オトイ","familyNameLang":"ja-Kana"},{"familyName":"Otoi","familyNameLang":"en"}],"givenNames":[{"givenName":"威重","givenNameLang":"ja"},{"givenName":"タケシゲ","givenNameLang":"ja-Kana"},{"givenName":"Takeshige","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"772","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"292974/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/292974/profile-ja.html"},{"nameIdentifier":"30311814","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=30311814"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-07-17"}],"displaytype":"detail","filename":"reth_24_25.pdf","filesize":[{"value":"956 KB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2011727/files/reth_24_25.pdf"},"version_id":"28b07b62-bc74-4154-935e-f9d3e96bdf48"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Human adipose tissuee–derived","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mesenchymal stem cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell preservation solution","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell aggregation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Oxygen partial pressure","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Relationship between oxygen partial pressure and inhibition of cell aggregation of human adipose tissue-derived mesenchymal stem cells stored in cell preservation solutions","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Relationship between oxygen partial pressure and inhibition of cell aggregation of human adipose tissue-derived mesenchymal stem cells stored in cell preservation solutions","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-07-17"},"publish_date":"2024-07-17","publish_status":"0","recid":"2011727","relation_version_is_last":true,"title":["Relationship between oxygen partial pressure and inhibition of cell aggregation of human adipose tissue-derived mesenchymal stem cells stored in cell preservation solutions"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-02-02T23:51:28.962781+00:00"}