{"created":"2025-01-14T01:33:02.207082+00:00","id":2012176,"links":{},"metadata":{"_buckets":{"deposit":"64b6d901-3868-4986-a1c3-8f3b1e181cd6"},"_deposit":{"created_by":7,"id":"2012176","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2012176"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02012176","sets":["1713853213384:1713853295607"]},"author_link":["1160"],"control_number":"2012176","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Substrate RNA specificity of ArcS","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-06-27","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageStart":"107505","bibliographicVolumeNumber":"300","bibliographic_titles":[{"bibliographic_title":"Journal of Biological Chemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Archaeosine (G+) is an archaea-specific tRNA modification synthesized via multiple steps. In the first step, archaeosine tRNA guanine transglucosylase (ArcTGT) exchanges the G15 base in tRNA with 7-cyano-7-deazaguanine (preQ0). In Euryarchaea, preQ015 in tRNA is further modified by archaeosine synthase (ArcS). Thermococcus kodakarensis ArcS catalyzes a lysine-transfer reaction to produce preQ0-lysine (preQ0-Lys) as an intermediate. The resulting preQ0-Lys15 in tRNA is converted to G+15 by a radical S-adenosyl-L-methionine enzyme for archaeosine formation (RaSEA), which forms a complex with ArcS. Here, we focus on the substrate tRNA recognition mechanism of ArcS. Kinetic parameters of ArcS for lysine and tRNA-preQ0 were determined using a purified enzyme. RNA fragments containing preQ0 were prepared from Saccharomyces cerevisiae tRNAPhe-preQ015. ArcS transferred 14C-labeled lysine to RNA fragments. Furthermore, ArcS transferred lysine to preQ0 nucleoside and preQ0 nucleoside 5′-monophosphate. Thus, the L-shaped structure and the sequence of tRNA are not essential for the lysine-transfer reaction by ArcS. However, the presence of D-arm structure accelerates the lysine-transfer reaction. Because ArcTGT from thermophilic archaea recognizes the common D-arm structure, we expected the combination of T. kodakarensis ArcTGT and ArcS and RaSEA complex would result in the formation of preQ0-Lys15 in all tRNAs. This hypothesis was confirmed using 46 T. kodakarensis tRNA transcripts and three Haloferax volcanii tRNA transcripts. In addition, ArcTGT did not exchange the preQ0-Lys15 in tRNA with guanine or preQ0 base, showing that formation of tRNA-preQ0-Lys by ArcS plays a role in preventing the reverse reaction in G+ biosynthesis.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Biochemistry and Molecular Biology","subitem_publisher_language":"en"},{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an open access article under the CC BY license.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"00219258","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1083351X","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA1202441X","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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タクヤ","creatorNameLang":"ja"},{"creatorName":"カワムラ, タクヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Kawamura, Takuya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヤマガミ, リョウタ","creatorNameLang":"ja"},{"creatorName":"ヤマガミ, リョウタ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamagami, Ryota","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"オカ, ナツヒサ","creatorNameLang":"ja"},{"creatorName":"オカ, ナツヒサ","creatorNameLang":"ja-Kana"},{"creatorName":"Oka, Natsuhisa","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"平田, 章","creatorNameLang":"ja"},{"creatorName":"ヒラタ, アキラ","creatorNameLang":"ja-Kana"},{"creatorName":"Hirata, Akira","creatorNameLang":"en"}],"familyNames":[{"familyName":"平田","familyNameLang":"ja"},{"familyName":"ヒラタ","familyNameLang":"ja-Kana"},{"familyName":"Hirata","familyNameLang":"en"}],"givenNames":[{"givenName":"章","givenNameLang":"ja"},{"givenName":"アキラ","givenNameLang":"ja-Kana"},{"givenName":"Akira","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1160","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"364635/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/364635/profile-ja.html"},{"nameIdentifier":"60527381","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=60527381"}]},{"creatorNames":[{"creatorName":"ヨコガワ, タカシ","creatorNameLang":"ja"},{"creatorName":"ヨコガワ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"Yokogawa, Takashi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ホリ, ヒロユキ","creatorNameLang":"ja"},{"creatorName":"ホリ, 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RNAs","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ArcS from Thermococcus kodakarensis transfers L-lysine to preQ0 nucleoside derivatives as minimum substrate RNAs","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-07-31"},"publish_date":"2024-07-31","publish_status":"0","recid":"2012176","relation_version_is_last":true,"title":["ArcS from Thermococcus kodakarensis transfers L-lysine to preQ0 nucleoside derivatives as minimum substrate RNAs"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-31T02:25:55.179942+00:00"}