| Item type |
文献 / Documents(1) |
| 公開日 |
2025-06-27 |
| アクセス権 |
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アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
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|
関連識別子 |
https://doi.org/10.1093/dnares/dsr022 |
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|
関連名称 |
10.1093/dnares/dsr022 |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
The Lifestyle of the Segmented Filamentous Bacterium : A Non-Culturable Gut-Associated Immunostimulating Microbe Inferred by Whole-Genome Sequencing |
| タイトル別表記 |
|
|
その他のタイトル |
Lifestyle of the SFB Inferred by Genome Sequencing |
| 著者 |
Kuwahara, Tomomi
Ogura, Yositoshi
Oshima, Kenshiro
Kurokawa, Ken
Ooka, Tadasuke
Hirakawa, Hideki
Itoh, Takehiko
Nakayama-Imaohji, Haruyuki
Ichimura, Minoru
Itoh, Kikuji
石舟, 智恵子
Maekawa, Yoichi
安友, 康二
Hattori, Masahira
Hayashi, Tetsuya
|
| 抄録 |
|
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内容記述 |
Numerous microbes inhabit the mammalian intestinal track and strongly impact host physiology; however, our understanding of this ecosystem remains limited owing to the high complexity of the microbial community and the presence of numerous non-culturable microbes. Segmented filamentous bacteria (SFBs), which are clostridia-related Gram-positive bacteria, are among such non-culturable populations and are well known for their unique morphology and tight attachment to intestinal epithelial cells. Recent studies have revealed that SFBs play crucial roles in the post-natal maturation of gut immune function, especially the induction of Th17 lymphocytes. Here, we report the complete genome sequence of mouse SFBs. The genome, which comprises a single circular chromosome of 1 620 005 bp, lacks genes for the biosynthesis of almost all amino acids, vitamins/cofactors and nucleotides, but contains a full set of genes for sporulation/germination and, unexpectedly, for chemotaxis/flagella-based motility. These findings suggest a triphasic lifestyle of the SFB, which comprises two types of vegetative (swimming and epicellular parasitic) phases and a dormant (spore) phase. Furthermore, SFBs encode four types of flagellin, three of which are recognized by Toll-like receptor 5 and could elicit the innate immune response. Our results reveal the non-culturability, lifestyle and immunostimulation mechanisms of SFBs and provide a genetic basis for the future development of the SFB cultivation and gene-manipulation techniques. |
| キーワード |
|
|
主題 |
segmented filamentous bacterium |
| キーワード |
|
|
主題 |
non-culturable bacteria |
| キーワード |
|
|
主題 |
genome |
| キーワード |
|
|
主題 |
flagella |
| 書誌情報 |
en : DNA Research
巻 18,
号 4,
p. 291-303,
発行日 2011-07-26
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| 収録物ID |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
17561663 |
| 収録物ID |
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|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
13402838 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11095219 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12072423 |
| 出版者 |
|
|
出版者 |
Kazusa DNA Research Institute |
| 出版者 |
|
|
出版者 |
Oxford University Press |
| 権利情報 |
|
|
権利情報 |
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| EID |
|
|
識別子 |
253053 |
| 言語 |
|
|
言語 |
eng |
dr_18_4_291.pdf (499 KB)
