| Item type |
文献 / Documents(1) |
| 公開日 |
2025-07-04 |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1016/j.fob.2014.06.002 |
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関連名称 |
10.1016/j.fob.2014.06.002 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Comprehensive enzymatic analysis of the amylolytic system in the digestive fluid of the sea hare, Aplysia kurodai : Unique properties of two α-amylases and two α-glucosidases |
| 著者 |
辻, 明彦
Nishiyama, Nami
Ohshima, Miki
Maniwa, Saori
Kuwamura, Shuji
Shiraishi, Masataka
湯浅, 恵造
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| 抄録 |
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内容記述 |
Sea lettuce (Ulva pertusa) is a nuisance species of green algae that is found all over the world. East-Asian species of the marine gastropod, the sea hare Aplysia kurodai, shows a clear feeding preference for sea lettuce. Compared with cellulose, sea lettuce contains a higher amount of starch as a storage polysaccharide. However, the entire amylolytic system in the digestive fluid of A. kurodai has not been studied in detail. We purified α-amylases and α-glucosidases from the digestive fluid of A. kurodai and investigated the synergistic action of these enzymes on sea lettuce. A. kurodai contain two α-amylases (59 and 80 kDa) and two α-glucosidases (74 and 86 kDa). The 59-kDa α-amylase, but not the 80-kDa α-amylase, was markedly activated by Ca2+ or Cl−. Both α-amylases degraded starch and maltoheptaose, producing maltotriose, maltose, and glucose. Glucose production from starch was higher with 80-kDa α-amylase than with 59-kDa α-amylase. Kinetic analysis indicated that 74-kDa α-glucosidase prefers short α-1,4-linked oligosaccharide, whereas 86-kDa α-glucosidase prefers large α-1,6 and α-1,4-linked polysaccharides such as glycogen. When sea lettuce was used as a substrate, a 2-fold greater amount of glucose was released by treatment with 59-kDa α-amylase and 74-kDa α-glucosidase than by treatment with 45-kDa cellulase and 210-kDa β-glucosidase of A. kurodai. Unlike mammals, sea hares efficiently digest sea lettuce to glucose by a combination of two α-amylases and two α-glucosidases in the digestive fluids without membrane-bound maltase–glucoamylase and sucrase–isomaltase complexes. |
| キーワード |
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|
主題 |
α-Amylase |
| キーワード |
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|
主題 |
α-Glucosidase |
| キーワード |
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|
主題 |
Sea hare |
| キーワード |
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|
主題 |
Sea lettuce |
| 書誌情報 |
en : FEBS Open Bio
巻 4,
号 1,
p. 560-570,
発行日 2014-06-18
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| 収録物ID |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
22115463 |
| 出版者 |
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出版者 |
Federation of the European Biochemical Societies |
| 出版者 |
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出版者 |
John Wiley & Sons |
| 出版者 |
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出版者 |
Elsevier |
| 権利情報 |
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|
権利情報 |
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
| EID |
|
|
識別子 |
282966 |
| 言語 |
|
|
言語 |
eng |
fob_4_1_560.pdf (928 KB)
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