{"created":"2025-02-19T05:22:09.543991+00:00","id":2012644,"links":{},"metadata":{"_buckets":{"deposit":"e90342b4-05c9-4af5-999b-6761c4384da5"},"_deposit":{"created_by":51,"id":"2012644","owner":"51","owners":[51],"pid":{"revision_id":0,"type":"depid","value":"2012644"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02012644","sets":["1713853213384:1713853295607"]},"author_link":["282","1116"],"control_number":"2012644","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Crystal Structure of T.H Protein Complex of GCS","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-04-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"24","bibliographicPageEnd":"18692","bibliographicPageStart":"18684","bibliographicVolumeNumber":"285","bibliographic_titles":[{"bibliographic_title":"Journal of Biological Chemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aminomethyltransferase, a component of the glycine cleavage system termed T-protein, reversibly catalyzes the degradation of the aminomethyl moiety of glycine attached to the lipoate cofactor of H-protein, resulting in the production of ammonia, 5,10-methylenetetrahydrofolate, and dihydrolipoate-bearing H-protein in the presence of tetrahydrofolate. Several mutations in the human T-protein gene are known to cause nonketotic hyperglycinemia. Here, we report the crystal structure of Escherichia coli T-protein in complex with dihydrolipoate-bearing H-protein and 5-methyltetrahydrofolate, a complex mimicking the ternary complex in the reverse reaction. The structure of the complex shows a highly interacting intermolecular interface limited to a small area and the protein-bound dihydrolipoyllysine arm inserted into the active site cavity of the T-protein. Invariant Arg292 of the T-protein is essential for complex assembly. The structure also provides novel insights in understanding the disease-causing mutations, in addition to the disease-related impairment in the cofactor-enzyme interactions reported previously. Furthermore, structural and mutational analyses suggest that the reversible transfer of the methylene group between the lipoate and tetrahydrofolate should proceed through the electron relay-assisted iminium intermediate formation.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Biochemistry and Molecular Biology","subitem_publisher_language":"en"},{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an Open Access article under the CC BY license.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"1083351X","subitem_source_identifier_type":"EISSN"},{"subitem_source_identifier":"00219258","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"AA1202441X","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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Nobuo","creatorNameLang":"en"}],"familyNames":[{"familyName":"真板","familyNameLang":"ja"},{"familyName":"マイタ","familyNameLang":"ja-Kana"},{"familyName":"Maita","familyNameLang":"en"}],"givenNames":[{"givenName":"宣夫","givenNameLang":"ja"},{"givenName":"ノブオ","givenNameLang":"ja-Kana"},{"givenName":"Nobuo","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"282","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00404046","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=00404046"}]},{"creatorNames":[{"creatorName":"Fujiwara, Kazuko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yoshizawa, Akiyasu C.","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakagawa, Atsushi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"谷口, 寿章","creatorNameLang":"ja"},{"creatorName":"タニグチ, ヒサアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Taniguchi, 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SUBSTRATE, DISEASE-RELATED MUTATIONS, AND REACTION MECHANISM","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"51","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-03-07"},"publish_date":"2025-03-07","publish_status":"0","recid":"2012644","relation_version_is_last":true,"title":["Crystal Structure of Aminomethyltransferase in Complex with Dihydrolipoyl-H-Protein of the Glycine Cleavage System : IMPLICATIONS FOR RECOGNITION OF LIPOYL PROTEIN SUBSTRATE, DISEASE-RELATED MUTATIONS, AND REACTION MECHANISM"],"weko_creator_id":"51","weko_shared_id":-1},"updated":"2025-03-07T05:08:14.026980+00:00"}