{"created":"2025-03-03T04:54:30.785351+00:00","id":2012777,"links":{},"metadata":{"_buckets":{"deposit":"99452895-5f1b-4e8f-996e-a6b7dc235dea"},"_deposit":{"created_by":121,"id":"2012777","owner":"121","owners":[121],"pid":{"revision_id":0,"type":"depid","value":"2012777"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02012777","sets":["1713853213384:1713853295607"]},"author_link":["680"],"control_number":"2012777","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-09-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"18","bibliographicPageStart":"dev.202079","bibliographicVolumeNumber":"150","bibliographic_titles":[{"bibliographic_title":"Development","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Chicken embryos are a powerful and widely used animal model in developmental biology studies. Since the development of CRISPR technology, gene-edited chickens have been generated by transferring primordial germ cells (PGCs) into recipients after genetic modifications. However, low inheritance caused by competition between host germ cells and the transferred cells is a common complication and greatly reduces production efficiency. Here, we generated a gene-edited chicken, in which germ cells can be ablated in a drug-dependent manner, as recipients for gene-edited PGC transfer. We used the nitroreductase/metronidazole (NTR/Mtz) system for cell ablation, in which nitroreductase produces cytotoxic alkylating agents from administered metronidazole, causing cell apoptosis. The chicken Vasa homolog (CVH) gene locus was used to drive the expression of the nitroreductase gene in a germ cell-specific manner. In addition, a fluorescent protein gene, mCherry, was also placed in the CVH locus to visualize the PGCs. We named this system ‘germ cell-specific autonomous removal induction’ (gSAMURAI). gSAMURAI chickens will be an ideal recipient to produce offspring derived from transplanted exogenous germ cells.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Company of Biologists","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"09501991","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"14779129","subitem_source_identifier_type":"EISSN"},{"subitem_source_identifier":"AA10667805","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA12024599","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1242/dev.202079"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1242/dev.202079","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"410813"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chen, Yi-Chen","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Saito, Daisuke","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Suzuki, Takayuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"竹本, 龍也","creatorNameLang":"ja"},{"creatorName":"タケモト, タツヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Takemoto, Tatsuya","creatorNameLang":"en"}],"familyNames":[{"familyName":"竹本","familyNameLang":"ja"},{"familyName":"タケモト","familyNameLang":"ja-Kana"},{"familyName":"Takemoto","familyNameLang":"en"}],"givenNames":[{"givenName":"龍也","givenNameLang":"ja"},{"givenName":"タツヤ","givenNameLang":"ja-Kana"},{"givenName":"Tatsuya","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"680","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"274209/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/274209/profile-ja.html"},{"nameIdentifier":"30443899","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=30443899"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-03-25"}],"displaytype":"detail","filename":"dev_150_18_dev202079.pdf","filesize":[{"value":"5.6 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2012777/files/dev_150_18_dev202079.pdf"},"version_id":"5e265b5c-5d5a-4ad2-80a4-86a9e08c7fbf"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Chicken primordial germ cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MAD7 nuclease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Nitroreductase/metronidazole","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell ablation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An inducible germ cell ablation chicken model for high-grade germline chimeras","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An inducible germ cell ablation chicken model for high-grade germline chimeras","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"121","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-03-25"},"publish_date":"2025-03-25","publish_status":"0","recid":"2012777","relation_version_is_last":true,"title":["An inducible germ cell ablation chicken model for high-grade germline chimeras"],"weko_creator_id":"121","weko_shared_id":-1},"updated":"2025-03-25T04:55:33.094966+00:00"}