{"created":"2025-03-06T01:22:38.752476+00:00","id":2012818,"links":{},"metadata":{"_buckets":{"deposit":"58a8df90-d49c-4766-b854-ea704a2bdcba"},"_deposit":{"created_by":51,"id":"2012818","owner":"51","owners":[51],"pid":{"revision_id":0,"type":"depid","value":"2012818"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02012818","sets":["1713853213384:1713853295607"]},"author_link":["1160"],"control_number":"2012818","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"tRNA recognition mechanism of E. coli TrmH","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-10-16","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"56","bibliographicPageStart":"43","bibliographicVolumeNumber":"175","bibliographic_titles":[{"bibliographic_title":"The Journal of Biochemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"TrmH is a eubacterial tRNA methyltransferase responsible for formation of 2’-O-methylguaosine at position 18 (Gm18) in tRNA. In Escherichia coli cells, only 14 tRNA species possess the Gm18 modification. To investigate the substrate tRNA selection mechanism of E. coli TrmH, we performed biochemical and structural studies. Escherichia coli TrmH requires a high concentration of substrate tRNA for efficient methylation. Experiments using native tRNA SerCGA purified from a trmH gene disruptant strain showed that modified nucleosides do not affect the methylation. A gel mobility-shift assay reveals that TrmH captures tRNAs without distinguishing between relatively good and very poor substrates. Methylation assays using wild-type and mutant tRNA transcripts revealed that the location of G18 in the D-loop is very important for efficient methylation by E. coli TrmH. In the case of tRNASer, tRNATyrand tRNALeu, the D-loop structure formed by interaction with the long variable region is important. For tRNAGln, the short distance between G18 and A14 is important. Thus, our biochemical study explains all Gm18 modification patterns in E. coli tRNAs. The crystal structure of E. coli TrmH has also been solved, and the tRNA binding mode of E. coli TrmH is discussed based on the structure.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"17562651","subitem_source_identifier_type":"EISSN"},{"subitem_source_identifier":"0021924X","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"AA12096002","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA00694073","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1093/jb/mvad076"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1093/jb/mvad076","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"404589"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kohno, Yoh","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ito, Asako","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Okamoto, Aya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamagami, Ryota","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"平田, 章","creatorNameLang":"ja"},{"creatorName":"ヒラタ, アキラ","creatorNameLang":"ja-Kana"},{"creatorName":"Hirata, Akira","creatorNameLang":"en"}],"familyNames":[{"familyName":"平田","familyNameLang":"ja"},{"familyName":"ヒラタ","familyNameLang":"ja-Kana"},{"familyName":"Hirata","familyNameLang":"en"}],"givenNames":[{"givenName":"章","givenNameLang":"ja"},{"givenName":"アキラ","givenNameLang":"ja-Kana"},{"givenName":"Akira","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1160","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"364635/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/364635/profile-ja.html"},{"nameIdentifier":"60527381","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=60527381"}]},{"creatorNames":[{"creatorName":"Hori, Hiroyuki","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-03-14"}],"displaytype":"detail","filename":"jb_175_1_43.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2012818/files/jb_175_1_43.pdf"},"version_id":"cd492c46-7541-46ed-b64c-41297b935107"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"tRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA modification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tRNA methyltransferase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SPOUT","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Escherichia coli","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Escherichia coli tRNA (Gm18) methyltransferase (TrmH) requires the correct localization of its methylation site (G18) in the D-loop for efficient methylation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Escherichia coli tRNA (Gm18) methyltransferase (TrmH) requires the correct localization of its methylation site (G18) in the D-loop for efficient methylation","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"51","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-03-14"},"publish_date":"2025-03-14","publish_status":"0","recid":"2012818","relation_version_is_last":true,"title":["Escherichia coli tRNA (Gm18) methyltransferase (TrmH) requires the correct localization of its methylation site (G18) in the D-loop for efficient methylation"],"weko_creator_id":"51","weko_shared_id":-1},"updated":"2025-03-14T02:02:23.652753+00:00"}