{"created":"2025-03-10T04:58:21.509586+00:00","id":2012853,"links":{},"metadata":{"_buckets":{"deposit":"2977a39e-c94c-438e-b2f6-e8496437ed5a"},"_deposit":{"created_by":8,"id":"2012853","owners":[8],"pid":{"revision_id":0,"type":"depid","value":"2012853"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02012853","sets":["1713853213384:1713853295607"]},"author_link":["1262","820","304"],"control_number":"2012853","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Inositol glycan from GIPC","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2025-03-07","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{"bibliographic_title":"The Journal of Biochemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Glycosylinositol phosphoceramide (GIPC) is the most abundant sphingolipids in plants. Previously, we found phospholipase D (PLD) activity that hydrolyzes GIPC to phytoceramide 1-phosphate (PCerP) in plants and revealed that GIPC-PLD activity is carried out by an enzyme encoded by non-specific phospholipase C3 (NPC3) gene. In this study, we established a method for isolation and quantification of inositol glycan (InoGly), a counterpart of PCerP produced from GIPC, using TLC imaging. We confirmed that A. thaliana NPC3 protein and partially purified GIPC-PLD from cabbage produced InoGly in a similar amount to that of PCerP from purified GIPC. We applied our method to determination of InoGly present in plant tissues and found that it was present at 40–80 nmol/g (wet wt.) in cabbage leaves, radish root and broccoli stem, and increased to 80–120 nmol/g after homogenization of the tissues. Similar increases in PCerP and decreases in GIPC were observed after homogenization, indicating that InoGly and PCerP were produced from GIPC by GIPC-PLD activity in response to homogenization. We believe our method, which does not require the complicated process or large device, will contribute to a better understanding of GIPC metabolism and signaling in plants.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press","subitem_publisher_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"17562651","subitem_source_identifier_type":"EISSN"},{"subitem_source_identifier":"0021924X","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"AA12096002","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA00694073","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"embargoed access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_f1cf"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Biochemistry following peer review. The version of record Md Majidul Islam, Rumana Yesmin Hasi, Yuta Umemura, Hide-Nori Tanaka, Yudai Kondo, Toshiki Ishikawa, Minoru Nagano, Hanif Ali, Ryushi Kawakami, Mutsumi Aihara, Tamotsu Tanaka, Method for isolation and quantification of inositol glycan produced by glycosylinositol phosphoceramide-hydrolyzing phospholipase D in plants, The Journal of Biochemistry, is available online at: https:// doi.org/10.1093/jb/mvaf013."}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1093/jb/mvaf013"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1093/jb/mvaf013","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"416967"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Islam, Md Majidul","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hasi, Rumana Yesmin","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Umemura, Yuta","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tanaka, Hide-Nori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kondo, 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D","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"non-specific phospholipase C3","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"inositol glycan","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phytoceramide 1-phosphate","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Method for isolation and quantification of inositol glycan produced by glycosylinositol phosphoceramide-hydrolyzing phospholipase D in plants","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Method for isolation and quantification of inositol glycan produced by glycosylinositol phosphoceramide-hydrolyzing phospholipase D in plants","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"8","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-03-28"},"publish_date":"2025-03-28","publish_status":"0","recid":"2012853","relation_version_is_last":true,"title":["Method for isolation and quantification of inositol glycan produced by glycosylinositol phosphoceramide-hydrolyzing phospholipase D in plants"],"weko_creator_id":"8","weko_shared_id":-1},"updated":"2025-03-28T09:13:44.121603+00:00"}