| Item type |
文献 / Documents(1) |
| 公開日 |
2025-07-11 |
| アクセス権 |
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|
アクセス権 |
embargoed access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_f1cf |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1002/cbic.202500323 |
|
|
関連名称 |
10.1002/cbic.202500323 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Regulation of Gene Expression of Mouse D-Amino Acid Oxidase |
| 著者 |
Trinh, Huong Thi Thanh
Shishido, Yuji
チャン, ホアンナム
Tran, Diem Hong
Kim, Soo Hyeon
Sogabe, Hirofumi
福井, 清
|
| 抄録 |
|
|
内容記述 |
D-amino acid oxidase (DAO) catalyzes oxidative deamination of D-amino acids, producing 2-oxo acids, hydrogen peroxide and ammonia. In mammals, DAO is essential for metabolizing both endogenous and exogenous D-amino acids. Our previous studies on human DAO identified two promoter regions (P1 and P2), a negative regulatory element in intron 1, and several transcription factor binding sites. In this study, we investigated the regulatory mechanism of mouse DAO gene expression to compare with the human system. To determine the promoter activity in the upstream region of initiation site, we constructed plasmids containing mouse DAO gene fragments inserted into pGL4 [luc2P/Hygro] vector and assessed luciferase activity in LLC-PK1 cells. We analyzed a series of deletion constructs, revealing promoter activity in all tested fragments. The highest promoter activity was detected in the -333/-87 subregion, with residual activities in the -87/+111 region. Bioinformatics analysis identified transcription factors, including NEUR, EGRF, ZF07, ZF11, KLFS, SP1F and ZF02, which bind to both the human and mouse DAO genes at conserved positions, suggesting their critical role in regulating DAO promoter activity. |
| キーワード |
|
|
主題 |
D-amino acid oxidase |
| キーワード |
|
|
主題 |
gene regulation |
| キーワード |
|
|
主題 |
transcription factors |
| キーワード |
|
|
主題 |
promoter activity |
| キーワード |
|
|
主題 |
neurodegeneration |
| 書誌情報 |
en : ChemBioChem
発行日 2025-06-18
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| 収録物ID |
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|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
14397633 |
| 収録物ID |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
14394227 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11497584 |
| 出版者 |
|
|
出版者 |
Wiley-VCH GmbH, Weinheim |
| 備考 |
|
|
値 |
This is the peer reviewed version of the following article: Huong Thi Thanh Trinh, Yuji Shishido, Nam Hoang Tran, Diem Hong Tran, Soo Hyeon Kim, Hirofumi Sogabe, Kiyoshi Fukui. (2025), Regulation of Gene Expression of Mouse D-Amino Acid Oxidase. ChemBioChem, which has been published in final form at https://doi.org/10.1002/cbic.202500323. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
| EID |
|
|
識別子 |
443433 |
| 言語 |
|
|
言語 |
eng |
cbic.pdf (2.9 MB)
