| Item type |
文献 / Documents(1) |
| 公開日 |
2025-08-25 |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1093/stcltm/szae006 |
|
|
関連名称 |
10.1093/stcltm/szae006 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Conditioned Medium From Stem Cells of Human Exfoliated Deciduous Teeth Alleviates Mouse Osteoarthritis by Inducing sFRP1-Expressing M2 Macrophages |
| 著者 |
Xia, Linze
加納, 史也
橋本, 登
Liu, Yao
Khurel-Ochir, Tsendsuren
Ogasawara, Naoko
Ding, Cheng
Xu, Yang
Hibi, Hideharu
岩﨑, 智憲
田中, 栄二
山本, 朗仁
|
| 抄録 |
|
|
内容記述 |
Intravenous administration of conditioned medium from stem cells of human exfoliated deciduous teeth (SHED-CM) regenerates mechanically injured osteochondral tissues in mouse temporomandibular joint osteoarthritis (TMJOA). However, the underlying therapeutic mechanisms remain unclear. Here, we showed that SHED-CM alleviated injured TMJ by inducing anti-inflammatory M2 macrophages in the synovium. Depletion of M2 by Mannosylated Clodrosome abolished the osteochondral repair activities of SHED-CM. Administration of CM from M2-induced by SHED-CM (M2-CM) effectively ameliorated mouse TMJOA by inhibiting chondrocyte inflammation and matrix degradation while enhancing chondrocyte proliferation and matrix formation. Notably, in vitro, M2-CM directly suppressed the catabolic activities while enhancing the anabolic activities of interleukin-1β-stimulated mouse primary chondrocytes. M2-CM also inhibited receptor activator of nuclear factor NF-κB ligand-induced osteoclastogenesis in RAW264.7 cells. Secretome analysis of M2-CM and M0-CM revealed that 5 proteins related to anti-inflammation and/or osteochondrogenesis were enriched in M2-CM. Of these proteins, the Wnt signal antagonist, secreted frizzled-related protein 1 (sFRP1), was the most abundant and played an essential role in the shift to anabolic chondrocytes, suggesting that M2 ameliorated TMJOA partly through sFRP1. This study suggests that secretome from SHED exerted remarkable osteochondral regeneration activities in TMJOA through the induction of sFRP1-expressing tissue-repair M2 macrophages. |
| キーワード |
|
|
主題 |
mesenchymal stem cell |
| キーワード |
|
|
主題 |
dental pulp stem cell |
| キーワード |
|
|
主題 |
osteoarthritis |
| キーワード |
|
|
主題 |
temporomandibular joint |
| キーワード |
|
|
主題 |
secretome of M2 macrophage |
| キーワード |
|
|
主題 |
osteochondral regeneration |
| キーワード |
|
|
主題 |
Wnt antagonist |
| 書誌情報 |
en : Stem Cells Translational Medicine
巻 13,
号 4,
p. 399-413,
発行日 2024-02-16
|
| 収録物ID |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
21576580 |
| 収録物ID |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
21576564 |
| 出版者 |
|
|
出版者 |
Oxford University Press |
| 権利情報 |
|
|
権利情報 |
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| EID |
|
|
識別子 |
408120 |
| 言語 |
|
|
言語 |
eng |
sctm_13_4_399.pdf (45.3 MB)
.png)
