| Item type |
文献 / Documents(1) |
| 公開日 |
2025-10-21 |
| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
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|
関連識別子 |
https://doi.org/10.1111/asj.70095 |
|
|
関連名称 |
10.1111/asj.70095 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Zinc Chloride Supplementation During In Vitro Fertilization Reduces Polyspermic Fertilization of Porcine Oocytes |
| 著者 |
Kaewma, Supitcha
音井, 威重
Widodo, Oky Setyo
長原, 恵
中井, 綾
Nguyen, Suong T.
Nakayama, Yuichiro
Tharasanit, Theerawat
平田, 真樹
谷原, 史倫
|
| 抄録 |
|
|
内容記述 |
As zinc is important for the fertilization competency of sperms and oocytes, we investigated the effects of zinc chloride (ZnCl2) supplementation during in vitro fertilization (IVF) on porcine oocyte fertilization and development. We evaluated the effects of ZnCl2 concentration (0, 1, 10, and 20 μg/mL) on the quality of frozen-thawed boar spermatozoa cultured for 5 h and on oocyte fertilization and embryo development after IVF. Spermatozoa from three different boars were additionally tested. ZnCl2 supplementation effects along with metal chelators, calcium ethylenediaminetetraacetic acid (Ca-EDTA) and zinc EDTA (Zn-EDTA), were also examined. ZnCl2 supplementation did not affect the quality of frozen-thawed spermatozoa after culture for 5 h. Supplementation with 1-μg/mL ZnCl2 decreased the percentage of polyspermic fertilization compared to that at 0- and 20-μg/mL ZnCl2. Moreover, it increased blastocyst formation rate compared to that in the other supplementation groups. In different boar spermatozoa, ZnCl2 supplementation (1 μg/mL) decreased polyspermic fertilization but did not improve embryo development. Co-incubation with Ca-EDTA did not reduce polyspermic fertilization, but Zn-EDTA co-incubation reduced polyspermic fertilization similar to that with ZnCl2 alone. In conclusion, 1-μg/mL ZnCl2 supplementation during IVF reduces polyspermic fertilization but may not improve embryo development. |
| キーワード |
|
|
主題 |
embryo development |
| キーワード |
|
|
主題 |
in vitro fertilization |
| キーワード |
|
|
主題 |
monospermy |
| キーワード |
|
|
主題 |
pig |
| キーワード |
|
|
主題 |
zinc |
| 書誌情報 |
en : Animal Science Journal
巻 96,
号 1,
p. e70095,
発行日 2025-08-27
|
| 収録物ID |
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|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
17400929 |
| 収録物ID |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
13443941 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11677214 |
| 出版者 |
|
|
出版者 |
Japanese Society of Animal Science |
| 出版者 |
|
|
出版者 |
Wiley |
| 権利情報 |
|
|
権利情報 |
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| EID |
|
|
識別子 |
454047 |
| 言語 |
|
|
言語 |
eng |
asj_96_1_e70095.pdf (317 KB)
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