WEKO3
アイテム
19F NMR-based solvent accessibility profiling reveals tryptophan ring-flip dynamics in a protein
https://tokushima-u.repo.nii.ac.jp/records/2013576
https://tokushima-u.repo.nii.ac.jp/records/2013576203a4331-cbe7-432d-95d8-f217e54bd7a4
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
prosci_34_10_e70307.pdf (1.8 MB)
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| アイテムタイプ | 文献 / Documents(1) | |||||||||||||||||||
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| 公開日 | 2025-10-22 | |||||||||||||||||||
| アクセス権 | ||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1002/pro.70307 | |||||||||||||||||||
| 関連名称 | 10.1002/pro.70307 | |||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | 19F NMR-based solvent accessibility profiling reveals tryptophan ring-flip dynamics in a protein | |||||||||||||||||||
| 著者 |
川越, 聡一郎
× 川越, 聡一郎
WEKO
1306
× Kumeta, Hiroyuki
× 齋尾, 智英
WEKO
1188
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| 抄録 | ||||||||||||||||||||
| 内容記述 | Aromatic ring flipping is a key component of protein conformational dynamics, particularly in facilitating breathing motions that involve cooperative rearrangements of hydrophobic cores. While phenylalanine and tyrosine flipping have been extensively studied, tryptophan flipping has often been overlooked due to assumptions about limited mobility arising from its bulkiness and the asymmetric structure of the indole ring. Here, we report a 19F NMR–based approach to probe the dynamic flipping of Trp23 in the DNA-binding domain (DBD) of heat shock factor 1 (Hsf1). We incorporated 5-fluorotryptophan into the Hsf1 DBD and monitored Trp23 flipping in solution using 19F NMR. Temperature-dependent analysis corroborated with relaxation dispersion experiments revealed interconversion between two conformers of Trp23, which correspond to the flip-in and flip-out states observed in crystal structures. Furthermore, D2O isotope shifts and solvent paramagnetic relaxation enhancement experiments revealed population shifts between the solvent-exposed flip-out state and the buried flip-in ground state. These results establish that Trp23 undergoes flipping between buried and solvent-exposed conformations in solution. This study represents the first application of 19F NMR-based solvent accessibility profiling to characterize aromatic ring flipping. The strategy presented here is broadly applicable to studies of aromatic dynamics in diverse protein systems. | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 主題 | 19F NMR | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 主題 | aromatic ring flipping | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 主題 | CPMG relaxation dispersion | |||||||||||||||||||
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| 主題 | protein breathing motion | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 主題 | solvent PRE | |||||||||||||||||||
| 書誌情報 |
en : Protein Science 巻 34, 号 10, p. e70307, 発行日 2025-09-13 |
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| 収録物識別子タイプ | EISSN | |||||||||||||||||||
| 収録物識別子 | 1469896X | |||||||||||||||||||
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| 収録物識別子タイプ | PISSN | |||||||||||||||||||
| 収録物識別子 | 09618368 | |||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||
| 収録物識別子 | AA12038287 | |||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||
| 収録物識別子 | AA10829011 | |||||||||||||||||||
| 出版者 | ||||||||||||||||||||
| 出版者 | Wiley Periodicals LLC. | |||||||||||||||||||
| 権利情報 | ||||||||||||||||||||
| 権利情報 | This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | |||||||||||||||||||
| EID | ||||||||||||||||||||
| 識別子 | 456333 | |||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||
