| Item type |
文献 / Documents(1) |
| 公開日 |
2025-11-26 |
| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.3390/ph18081099 |
|
|
関連名称 |
10.3390/ph18081099 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Atractylodes lancea (Thunb.) DC. [Asteraceae] Rhizome-Derived Exosome-like Nanoparticles Suppress Lipopolysaccharide-Induced Inflammation by Reducing Toll-like Receptor 4 Expression in BV-2 Murine Microglial Cells |
| 著者 |
Hyodo, Mizusa
川田, 敬
Ishida, Tomoaki
(井澤)石澤, 有紀
Matoba, Ryoko
Okamoto, Rina
Jobu, Kohei
堀川, 伊和
相澤, 風花
八木, 健太
新村, 貴博
Kawano, Yayoi
阿部, 真治
Hamada, Yukihiro
合田, 光寛
石澤, 啓介
|
| 抄録 |
|
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内容記述 |
Background/Objectives: Atractylodes lancea (Thunb.) DC. [Asteraceae] (ALR)-derived exosome-like nanoparticles (ALR-ELNs) exhibit anti-neuroinflammatory effects in microglial cells. However, the associated mechanisms and pathways are unknown. We aimed to characterize the effects of ALR-ELNs on inflammatory responses of BV-2 microglial cells to lipopolysaccharide (LPS) using RNA sequencing. Methods: ALR-ELNs were fractionated from ALR. BV-2 microglial murine cells were stimulated with LPS after treatment with ALR-ELNs. RNA sequencing was performed to analyze variations in mRNA levels. Ingenuity pathway analysis (IPA) was performed to investigate the mechanism of action of ALR-ELNs. mRNA expression was assessed using real-time quantitative polymerase chain reaction (qPCR). Results: The expression of 651 genes was downregulated, whereas that of 1204 genes was upregulated in LPS-stimulated BV2 cells pretreated with ALR-ELNs. The IPA showed that the effects of ALR-ELNs on inflammation took place through pathogen-influenced signaling. Network analysis via IPA showed that the Toll-like receptor (TLR) is involved in the suppression of inflammation by ALR-ELNs. The qPCR analysis showed that pretreatment with ALR-ELNs significantly reduced TLR4 mRNA expression. Conclusions: ALR-ELNs suppress the release of inflammatory mediators by downregulating TLR4 expression, which is a novel mechanism by which ALR-ELNs act on microglia. Identifying active ingredients in ALR-ELNs that downregulate TLR4 expression can advance the development of therapeutic drugs for neuroinflammatory diseases. |
| キーワード |
|
|
主題 |
Atractylodes lancea rhizome |
| キーワード |
|
|
主題 |
microglia |
| キーワード |
|
|
主題 |
exosome-like nanoparticle |
| キーワード |
|
|
主題 |
neuroinflammation |
| キーワード |
|
|
主題 |
RNA sequencing |
| キーワード |
|
|
主題 |
ingenuity pathway analysis |
| キーワード |
|
|
主題 |
Toll-like receptor 4 |
| キーワード |
|
|
主題 |
BV-2 |
| キーワード |
|
|
主題 |
nitric oxide |
| 書誌情報 |
en : Pharmaceuticals
巻 18,
号 8,
p. 1099,
発行日 2025-07-24
|
| 収録物ID |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
14248247 |
| 出版者 |
|
|
出版者 |
MDPI |
| 権利情報 |
|
|
権利情報 |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| EID |
|
|
識別子 |
447692 |
| 言語 |
|
|
言語 |
eng |
ph_18_8_1099.pdf (2.3 MB)
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