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Preventive Effect of Daiokanzoto (TJ-84) on 5-Fluorouracil-Induced Human Gingival Cell Death through the Inhibition of Reactive Oxygen Species Production
https://tokushima-u.repo.nii.ac.jp/records/2000104
https://tokushima-u.repo.nii.ac.jp/records/200010411c72e96-0b88-40e7-b7df-7eabdccbe71b
| 名前 / ファイル | ライセンス | アクション |
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pone_9_11_e112689.pdf (1.5 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||
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| 公開日 | 2024-09-25 | |||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1371/journal.pone.0112689 | |||||||||||||||||||||
| 関連名称 | 10.1371/journal.pone.0112689 | |||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||
| タイトル | ||||||||||||||||||||||
| タイトル | Preventive Effect of Daiokanzoto (TJ-84) on 5-Fluorouracil-Induced Human Gingival Cell Death through the Inhibition of Reactive Oxygen Species Production | |||||||||||||||||||||
| タイトル別表記 | ||||||||||||||||||||||
| その他のタイトル | TJ-84 Reduces 5-FU-Induced Cell Death in the Gingiva | |||||||||||||||||||||
| 著者 |
吉田, 賀弥
× 吉田, 賀弥
WEKO
1211
× 吉岡, 昌美× 岡村, 裕彦× Moriyama, Satomi
× 川添, 和義× Grenier, Daniel
× 日野出, 大輔
WEKO
1666
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| 抄録 | ||||||||||||||||||||||
| 内容記述 | Daiokanzoto (TJ-84) is a traditional Japanese herbal medicine (Kampo formulation). While many Kampo formulations have been reported to regulate inflammation and immune responses in oral mucosa, there is no evidence to show that TJ-84 has beneficial effects on oral mucositis, a disease resulting from increased cell death induced by chemotherapeutic agents such as 5-fluorouracil (5-FU). In order to develop effective new therapeutic strategies for treating oral mucositis, we investigated (i) the mechanisms by which 5-FU induces the death of human gingival cells and (ii) the effects of TJ-84 on biological events induced by 5-FU. 5-FU-induced lactate dehydrogenase (LDH) release and pore formation in gingival cells (Sa3 cell line) resulted in cell death. Incubating the cells with 5-FU increased the expression of nucleotide-binding domain and leucine-rich repeat containing PYD-3 (NLRP3) and caspase-1. The cleavage of caspase-1 was observed in 5-FU-treated cells, which was followed by an increased secretion of interleukin (IL)-1β. The inhibition of the NLRP3 pathway slightly decreased the effects of 5-FU on cell viability and LDH release, suggesting that NLRP3 may be in part involved in 5-FU-induced cell death. TJ-84 decreased 5-FU-induced LDH release and cell death and also significantly inhibited the depolarization of mitochondria and the up-regulation of 5-FU-induced reactive oxygen species (ROS) and nitric oxide (NO) production. The transcriptional factor, nuclear factor-κB (NF-κB) was not involved in the 5-FU-induced cell death in Sa3 cells. In conclusion, we provide evidence suggesting that the increase of ROS production in mitochondria, rather than NLRP3 activation, was considered to be associated with the cell death induced by 5-FU. The results also suggested that TJ-84 may attenuate 5-FU-induced cell death through the inhibition of mitochondrial ROS production. | |||||||||||||||||||||
| 書誌情報 |
en : PLOS ONE 巻 9, 号 11, p. e112689, 発行日 2014-11-12 |
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| 収録物識別子タイプ | EISSN | |||||||||||||||||||||
| 収録物識別子 | 19326203 | |||||||||||||||||||||
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| 出版者 | PLOS | |||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||
| 権利情報 | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||||||||||||||||||
| EID | ||||||||||||||||||||||
| 識別子 | 286472 | |||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||
